Error Reduction in Automated Gene Synthesis

描述(由申请人提供)： 基因的获取和操纵是生物学研究的核心。快速、准确的基因合成可以降低成本，加快研究的速度，因为这让科学家可以专注于实验，而不是花费时间来“剪切和粘贴”DNA。此外，它还有可能为“定向进化”技术提供原材料，从而使产生新生物功能的新方法成为可能。目前，低成本基因合成的主要障碍之一是在化学合成过程中经常引入序列错误。这项研究的目的是开发和实施新的技术，以消除合成DNA中的序列错误，并提高GONE合成过程的保真度。如果成功，该项目将显著降低基因合成成本，减少交付时间，并提高过程的可靠性。反过来，这些改进将导致基因合成在商业和NIH支持的研究中得到更广泛的使用，并显著提高研究生产率。我们计划通过我们现有的基因合成业务将这一过程商业化。错误减少技术将允许更短的交付时间和更低的基因合成成本，并使生产高质量的Gone变体文库成为可能。此外，我们产生的一些技术可能会带来新的产品，用于突变检测，用于生产非常高质量的寡核苷酸或用于高保真的PCR克隆。

DESCRIPTION (provided by applicant): The acquisition and manipulation of genes is at the heart of biological research. Fast, accurate gene synthesis can reduce the cost and accelerate the speed cf research by allowing scientists to focus on experiments rather than spending time "cutting and pasting'' DNA. In addition, it has the potential to enable novel approaches to generating new biological functions by providing the raw material for "directed-evolution" techniques. Currently one of the main barriers to low-cost gene synthesis is the frequent introduction of sequence errors during the course of chemical synthesis. The aim of this research is to develop and implement novel techniques that will remove sequence errors from synthetic DNA and increase the fidelity of the gone synthesis process. If successful, this project will significantly lower the cost of gene synthesis, reduce delivery times, and increase the reliability of the process. In turn, these improvements will lead to more widespread use of gene synthesis in commercial and NIH-supported research and measurable increases in research productivity. We plan to commercialize the process through our existing gene synthesis business. Error reduction technology will allow shorter delivery time and lower costs for gene synthesis and enable the production of high quality libraries of gone variants. In addition, a number of the technologies we generate may lead to new products for mutation detection, for the production of very high quality oligonucleotides or for high fidelity PCR cloning.