Effect of G-CSFR expression in bladder cancer

描述（由申请人提供）：越来越多的分子标记被发现与癌症转移或不良预后相关。通常，这些标记物是基于生物活性的潜力来检测的，这种生物活性会促进癌细胞的生长和转移。也有报道称，各种细胞因子或生长因子受体通过自分泌或旁分泌环组成性激活，从而支持癌细胞生长。虽然粒细胞集落刺激因子（G-CSF）表达已被证明在多种癌症细胞类型中表达，但越来越多的关于G-CSF受体（G-CSFR）在各种非造血人类癌症和癌细胞系中的异常表达的报道正在发表。我们的目的是清楚地证明移行细胞癌（TCC）中G-CSF表达与G-CSFR异常表达的结合可以作为更具侵袭性行为的预测因子。我们将通过小鼠TCC模型证明G-CSF/G-CSFR在体外和体内表达的生物学影响。因此，在越来越多的实体肿瘤中，G-CSFR的异常表达既可以作为影响临床结果的特定肿瘤细胞能力的标志，也可以作为许多这些肿瘤增强功能的机制。

DESCRIPTION (provided by applicant): An increasing number of molecular markers are being identified that correlate with cancer metastasis or a poor prognosis. Typically these markers were examined based on the potential for a biologic activity that would act to enhance cancer cell growth and metastasis. There are also reports of various cytokine or growth factor receptors that are constitutively activated via autocrine or paracrine loops thus supporting cancer cell growth. While granulocyte colony-stimulating factor (G-CSF) expression has been shown for a wide variety of cancer cell types, an increasing number of reports are being published regarding the aberrant expression of the G-CSF receptor (G-CSFR) by various non-hematopoietic human cancers and cancer cell lines. Our aim is to clearly demonstrate that G-CSF expression coupled with aberrant G-CSFR expression by transitional cell carcinoma (TCC) can be used as a predictor of more aggressive behavior. We will demonstrate the biological impact of G-CSF/G-CSFR expression in in vitro and also in vivo by using a murine TCC model. Thus the aberrant G-CSFR expression that is being demonstrated by an expanding variety of solid tumors may serve as both a marker of specific tumor cell capabilities impacting clinical outcome and as a mechanism for many of these tumor-enhancing functions.