ROLE OF EOSINOPHILS IN IMMUNITY TO S STERCORALIS

这项拨款提案的目标是确定以下方面的作用： 嗜酸性粒细胞在小鼠对线虫的先天性和适应性免疫应答中的作用 寄生虫粪类圆线虫在以前的研究中，我们已经表明， 对这种寄生虫的保护性免疫依赖于IL-5和嗜酸性粒细胞。 这份拨款提案将使用多种方法来描述 嗜余弦粒细胞参与免疫包括：（1）使用细胞因子， 嗜酸性粒细胞颗粒产物转基因（TG）和敲除小鼠（KO）小鼠（2） 使用TG和KO小鼠的组合遗传学，以及（3）测试和 定量鼠嗜酸性粒细胞颗粒产物在先天性 适应性免疫反应本项目的具体目标是：（1） 确定嗜酸性粒细胞在杀死幼虫S.斯氏 先天免疫反应在本节中，我们将确定是否杀死幼虫 S.粪肠球菌的先天免疫应答依赖于颗粒释放 嗜酸性粒细胞在幼虫的直接环境， 特异性小鼠嗜酸性粒细胞颗粒产物能够杀死 体外和体内。(2)为了确定嗜酸性粒细胞在诱导 对S.粪虫三个角色 将评价嗜酸性粒细胞在诱导免疫应答中的作用， 能够杀死和溶解幼虫抗原呈递的细胞毒性细胞 细胞和细胞因子产生细胞。(3)为了确定嗜酸性粒细胞在 通过适应性免疫反应杀死寄生虫。在这最后一节中 我们将确定嗜酸性粒细胞如何参与杀死幼虫， 与IgM和补体协作。总之，在这项研究中，我们 将决定嗜酸性粒细胞如何在保护性免疫反应中发挥作用， S.此外，我们将确定这些细胞在 从先天免疫反应向适应性免疫反应的转变。

The goal of this grant proposal is to determine the role of eosinophils in the innate and adaptive immune response of mice to the nematode parasite Strongyloides stercoralis. In previous studies we have shown that protective immunity to this parasite is dependent on IL-5 and on eosinophils. This grant proposal will use a variety of methods to describe the mechanism of cosinophil involvement in immunity including: (1) the use of cytokine and eosinophil granule product transgenic (TG) and knockout mice (KO) mice (2) the use of combinatorial genetics with the TG and KO mice and (3) the testing and quantifying of the role of murine eosinophil granule products in the innate and the adaptive immune response. The specific goals of this project are: (1) To determine the role of eosinophils in killing larval S. stercoralis by the innate immune response. In this section we will determine if killing of larval S. stercoralis by the innate immune response is dependent on granule release from the eosinophils in the immediate environment of the larvae and which specific mouse eosinophil granule products are capable of killing larvae in vitro and in vivo. (2) To determine the role of eosinophils in the induction of the adaptive immune response to larval S. stercoralis. Three roles for eosinophils in the induction of the immune response will be evaluated, cytotoxic cells capable of killing and lysing the larvae antigen presenting cells and cytokine producing cells. (3) To determine the role of eosinophils in killing parasites through the adaptive immune response. In this final section we will determine how eosinophils participate in killing larvae in collaboration with IgM and complement. In conclusion, in this proposed study we will determine how eosinophils contribute in the protective immune response to S. stercoralis and in addition we will determine the role of these cells in the transition from the innate to the adaptive immune response to this infection.