REGULATION OF PROLACTIN GENE EXPRESSION

描述：(改编自《调查员摘要》)：具体目标 包括：1)测试SLB的作用，SLB是一种假定的新的调节共激活剂 催乳素转录。2)评估其功能意义 利用遗传筛选鉴定Pit-1与CBP的相互作用 破坏CBP结合的Pit-1 POU结构域突变。3)确定是否 CAMP和TRH信号通路导致染色质结构的变化 PRL基因。4)确定是否刺激催乳素转录 涉及到转录共激活因子的招募的变化或 组蛋白修饰中的共抑制因子和变化。5)测试以下角色 特定的转录因子、共激活因子和共抑制因子参与 组织细胞的染色质结构和转录潜能 催乳素基因。这将涉及到使用活体内的 非洲爪哇卵母细胞系统以及使用果蝇胚胎系统的体外实验。

DESCRIPTION: (Adapted from the Investigator's Abstract): The specific aims include: 1) Test the role of SLB, a putative new co-activator in the regulation of prolactin transcription. 2) Evaluate the functional significance of the interaction of Pit-1 with CBP through the use of a genetic screen to identify mutations in the Pit-1 POU domain which disrupt bindind to CBP. 3) Determine if the cAMP and TRH signaling pathways lead to changes in the chromatin structure of the PRL gene. 4) Determine if stimulation of prolactin transcription involves changes in the recruitment of transcriptional co-activators or co-repressors and changes in histone modification. 5) Test the role that specific transcription factors, co-activators and co-repressors play in organizing the chromatin structure and transcriptional potential of the prolactin gene. This will involve chromatin reconstitution using an in vivo Xenopus oocyte system as well in vitro using a Drosophila embryo system.