M. genitalium: behavioral and reproductive correlates

描述(由申请人提供)： 生殖支原体是一种性传播微生物，最近通过聚合酶链式反应技术从阴道和宫颈标本中鉴定出来，患病率为3-5%。这种微生物能够侵入上生殖道，因此很可能是早产和其他妊娠并发症的原因。然而，生殖支原体在早产中的作用还没有得到系统的评价。我们建议研究秘鲁利马孕妇中生殖支原体感染与行为、感染和生殖的相关性。我们的研究设计将利用正在进行的早产感染原因的病例对照研究。750例早产病例和750例对照将从利马大型第三转诊中心--母婴围产期研究所的分娩中挑选出来。生殖支原体、沙眼衣原体和淋病奈瑟菌将在分娩后通过聚合酶链式反应获得宫颈拭子。我们还将评估阴道毛滴虫和细菌性阴道病。人口统计和行为信息将通过标准化访谈收集。本研究的具体目的如下：1.探讨生殖道支原体感染和其他性传播疾病与自然早产的关系。2.探讨秘鲁妇女孕期性史和行为特征与生殖支原体感染及其他性传播疾病的危险因素。这项研究将主要在秘鲁利马与马里兰州佩德罗·加西亚合作进行，作为国家卫生研究院资助HD 41682的延伸。

DESCRIPTION (provided by applicant): Mycoplasma genitalium is a sexually transmitted microorganism recently identified from vaginal and cervical specimens using PCR technology, with a prevalence of 3-5%. This microbe is capable of invading the upper genital tract, and thus could plausibly be a cause of preterm birth and other pregnancy complications. However, the role of M. genitalium in preterm birth has not been systematically evaluated. We propose to examine the behavioral, infectious and reproductive correlates of M. genitalium infection among pregnant women in Lima, Peru. Our study design will take advantage of an on-going case-control study of infectious causes of preterm birth. 750 preterm cases and 750 controls will be selected from deliveries at the Instituto Materno Perinatal, a large tertiary referral center in Lima. Cervical swabs will be obtained after delivery for M. genitalium by PCR, as well as Chlamydia trachomatis and Neisseria gonorrhoeae. We will also evaluate vaginal Trichomonas and bacterial vaginosis. Demographic and behavioral information will be collected by standardized interview. The study has the following specific aims: 1. To examine the associations between M.genitalium infection and other STD with spontaneous preterm birth. 2. To examine sexual history and behavioral characteristics as risk factors for M. genitalium and other STD in pregnancy among Peruvian women. This research will be conducted primarily in Lima, Peru in collaboration with Pedro Garcia, MD, as an extension of NIH grant HD 41682.