Use of Mouse Chimeras to Test Gene Function

MusWorks Inc.的商业目标是为生物医学研究界提供小鼠聚集嵌合体生产服务。小鼠嵌合体是基因复合动物，其细胞来自不同的胚胎。嵌合体分析是一种特别有价值的工具，可用于评估突变如何影响构成复杂生物系统基础的细胞相互作用，尤其是大脑的形成和功能。 我们的目的是评估的可行性和实用性，使用大量的嵌合体来分析各种基因，在中枢神经系统的发育和功能有不同的作用。我们将创建四个系列的嵌合体纳入不同的小鼠突变模型：时钟突变，普雷斯廷敲除小鼠，他莫昔芬诱导CREB-阻遏物转基因小鼠，和Math 1基因敲入。在应用嵌合体分析研究各种神经科学问题时，我们将通过扩展来评估嵌合体分析对一般生物医学研究的价值。大规模嵌合体分析有可能大大有助于分析小鼠突变表型，包括重要的人类功能和功能障碍的小鼠模型。

The commercial objective of MusWorks Inc. is to provide a mouse aggregation chimera production service to the biomedical research community. Mouse chimeras are genetically composite animals whose cells are derived from different embryos. Chimera analysis is a particularly valuable tool for assessing how mutations affect the cellular interactions that underlie complex biological systems, most notably the formation and function of the brain. We aim to evaluate the feasibility and utility of using large series of chimeras to analyze a variety of genes that have different roles in central nervous system development and function. We will create four series of chimeras incorporating different mouse mutant models: the Clock mutant, prestin knockout mice tamoxifen-inducible CREB-repressor transgenic mice, and a Math1 gene knock-in. In applying chimera analysis to study a variety of neuroscience problems, we will assess, by extension, the value of chimera analysis to biomedical research in general. Large-scale chimera analysis has the potential to contribute substantially to the analysis of mouse mutant phenotypes, including important mouse models of human function and dysfunction.