Association of Caries and Salivary Analytes

龋齿是一种多因素疾病，菌斑生物膜微生物的毒力、唾液功能和饮食营养等因素在龋齿发病中起着重要作用。我们假设，哺乳动物和非哺乳动物的唾液成分都与龋齿的发生风险有关。据推测，没有哪一种唾液成分比其他成分更重要，相反，这些成分中的每一种都以协调一致的方式相互作用，累积效应对预防龋齿很重要。我们将开发三明治酶联免疫测定法（ELISA）来定量唾液黏液蛋白MG1和MG2的水平；酸性富脯氨酸蛋白1和3，碱性富脯氨酸蛋白PRB-1和PRB-2；富脯氨酸糖基化蛋白；胱抑素S和SN；statherin;组他汀类药物1、3、5；溶菌酶;乳铁蛋白和过氧化物酶；微生物酶葡萄糖基转移酶和变形链球菌的葡萄糖基转移酶；而食品防腐剂中苯甲酸盐的用量很大。我们已经积累了4000多个静息全唾液样本。获得的数据将用于分析分析物水平的受试者内部稳定性；被试分析物水平的衰减、缺失、填充表面（DMFS）指数的横截面关联；以及DMFS与唾液分析物水平的纵向关联。对多种唾液成分的综合调查和聚类分析应产生有用的数据集，以测试这些成分与龋齿经历的关联，并确定表明龋齿风险增加的参数。该研究还将提供有用的信息，以确定唾液中苯甲酸酯水平与龋齿经历之间是否存在临床关系。

Dental caries is a multi-factorial disease in which the virulence of microbes in the plaque biofilm, salivary function and diet and nutrition play important roles. We hypothesize that salivary components of both mammalian and non-mammalian origin are associated with risk for the development of dental caries. It is speculated that no single salivary component is important than the others, rather each of these components interact in a concerted fashion and a cumulative effect is important in protecting against caries. We will develop sandwich enzyme-linked immunoassays (ELISA) to quantitate the levels of salivary mucins MG1 and MG2; acidic proline- rich proteins 1 and 3, basic proline-rich proteins PRB-1 and PRB-2; glycosylated proline-rich protein PRG; cystatins S and SN; statherin; histatins 1, 3 and 5; lysozyme; lactoferrin and peroxidase; microbial enzymes glucosyltransferase and glucosyltransferase from Streptococcus mutans; and food preservative benzoate in a large number of subjects. We have already accumulated more than 4000 samples of resting whole saliva. The data obtained will be analyzed for the intra-subject stability of the analyte levels; the cross-sectional association of the decay, missing, filled surfaces (DMFS) index of the subjects analyte levels; and the longitudinal association of the DMFS with salivary analyte levels. The comprehensive survey and the cluster analysis of multiple salivary components should generate a useful data set to test for the association of these components with the caries experience and to identify parameters that will indicate increased risk for dental caries. This study will also provide useful information to determine whether there is a clinical relationship between benzoate levels in saliva and caries experience.