INGESTIVE BEHAVIOR IN SEROTONIN RECEPTOR MUTANT MICE

描述：（改编自研究者摘要）此应用程序是一个 申请独立科学家奖（K 02），以促进研究事业 劳伦斯·特科特博士的发展特科特博士的长期目标是应用 分子遗传学方法来确定个人的贡献 5-羟色胺受体亚型对肾上腺素能调节复合物 行为。这项工作的一个主要重点将是发展遗传和 行为方法来确定神经机制，通过这些机制， 5-羟色胺5-HT 2C和5-HT 1A受体亚型调节食物摄入。初步 有证据表明5-HT 2C受体突变小鼠的摄食失调， 导致肥胖症的发展。5-HT 1A受体的初步研究 突变小鼠提出了这些动物大脑过度活跃的可能性 血清素系统5-HT 2C和5-HT 1A受体对大鼠脑缺血再灌注损伤的影响 调节进食的神经心理过程与 它们对参与调节的神经递质系统的影响 摄食行为 独立科学家奖将使博士Tecott发展新的技能， 追求他的研究目标的方法。例如， 研究计划是开发小鼠的摄食行为测定法。 这些努力将促进转基因技术应用于 研究喂养，使博士Tecott获得定量的赞赏 评估行为的方法。另一个重要目标是 研究计划是开发产生5-HT受体的程序 这些突变仅限于特定的大脑区域， 识别细胞类型。这种方法将有助于识别神经元 5-HT受体通过这些结构影响复杂的行为。这些 将在杰出合作者的指导下追求目标， Tecott博士开发的专业知识，将补充他现有的 神经解剖学和分子生物学技能。

DESCRIPTION: (Adapted from the Investigator's Abstract) This application is a request for an Independent Scientist Award (K02) to further the research career development of Dr. Laurence Tecott. Dr. Tecott's long term goal is to apply molecular genetic approaches to determine the contributions of individual serotonin receptor subtypes to the serotonergic regulation of complex behaviors. A major focus of this work will be the development of genetic and behavioral approaches for determining the neural mechanisms through which the serotonin 5-HT2C and 5-HT1A receptor subtypes regulate food intake. Preliminary evidence reveals a dysregulation of feeding in 5-HT2C receptor mutant mice, leading to the development of obesity. Preliminary studies of 5-HT1A receptor mutant mice raise the possibility that these animals have hyperactive brain serotonin systems. The impact of 5-HT2C and 5-HT1A receptors on the neuropsychological processes that regulate feeding will be correlated with their effects on neurotransmitter systems implicated in the regulation of ingestive behavior. An Independent Scientist Award will allow Dr. Tecott to develop new skills and approaches for pursuing his research goals. For example, a major focus of the research plan is the development of ingestive behavior assays for the mouse. These efforts will facilitate the application of transgenic technologies to the study of feeding and enable Dr. Tecott to gain an appreciation of quantitative approaches to the assessment of behavior. Another important goal of the research plan is the development of procedures for generating 5-HT receptor mutations that are restricted to particular brain regions and neurochemically identified cell types. Such approaches will be useful for identifying neural structures through which 5-HT receptors influence complex behaviors. These goals will be pursued with guidance from outstanding collaborators, allowing Dr. Tecott to develop expertise that will complement his existing neuroanatomical and molecular biology skills.