PROTEIN AND HISTOLOGY MINICORE GRANT FOR VISION RESEARCH

描述(由申请人提供)：密苏里大学眼科视觉研究学院建议建立两个研究模块，以支持该系正在进行的活动。蛋白质表达和分析模块(模块1)将在Sharma博士的指导下建立，用于晶体蛋白的表达、分离和分析。该模块将支持研究人员的需求，他们将需要大量的晶状体蛋白，在分析晶状体或视网膜成分方面需要帮助，以及需要培养整个晶状体或晶状体上皮细胞。晶状体上皮细胞在培养中的建立将使我们能够研究体内条件下的信号转导和基因表达。蛋白质分析模块的建立将使我们能够共享现有的资源，并提高我们为新加入的教职员工和我们计划根据任务增强计划招聘的新故障提供支持的能力。组织学和图像分析模块(模块2)将用于分析来自转基因动物或病理标本或供体组织的眼组织样本。这个模块将由Reneker博士指导。它将促进新闻部内NEI支持的项目之间的持续合作。此外，该模块将为我们的新教员发起的研究项目提供急需的支持。因此，这项建议的主要目标是： A)加强密苏里大学医学院视觉科学研究的研究基础设施， B)增加联合研究项目的机会，并为联合项目提供后勤支持； C)支持新视野研究人员的需求。

DESCRIPTION (provided by applicant): The vision research faculty in the Department of Ophthalmology at the University of Missouri propose to establish two research modules to support the ongoing activities in the department. The Protein expression and analysis module (Module #1) will be setup under the direction of Dr. Sharma for crystallin expression, isolation, and analysis. This module will support the need of investigators who will need large quantities of crystallins, need assistance in the analysis of lens or retinal constituents and the need to culture of whole lenses or lens epithelial cells. The establishment of lens epithelial cells in culture will allow us to look into signal transduction and gene expression under in vivo conditions. The establishment of protein analysis module will allow us to share the existing resources and increase our ability to offer support to recently joined faculty and the new faulty we plan on recruiting under the mission enhancement program. The Histology and image analysis module (Module #2) will be a facility for analyzing eye tissue samples from transgenic animals or pathologic specimens or donor tissues. This module will be directed by Dr. Reneker. It will foster the ongoing collaboration between the NEI supported projects within the department. In addition, the module will offer the much needed support for the research projects initiated by our new faculty. Therefore the main objectives of this proposal are to: a) enhance research infrastructure for vision science research at University of Missouri School of Medicine, b) enhance the opportunities for joint research projects and provide logistical support for joint projects, c) support the needs of new vision research faculty.