Cancer Center Tumor Phenotyping Core

描述（由申请人提供）： 1996年，俄亥俄州的癌症死亡率在50个州中排名第13位。 州和哥伦比亚特区。 俄亥俄州死亡率排名第16位 第12位是结直肠癌，第6位是乳腺癌。 死亡辛辛那提及其周边地区是美国 黑人、白人、男性和女性的癌症死亡率。汉密尔顿县 (site（辛辛那提大学的）具有统计学显著性较高的 癌症发病率高于俄亥俄州整体。这些数据点燃了临床医生， 研究人员和管理人员开发合作计划， 激发对癌症基础生物学的新研究， 将这些新知识转化为临床护理。这项建议 是一系列讨论小组的直接成果之一， 包括临床医生和基础研究人员，制定计划战略， 满足这些临床需求。临床/转化和基础研究人员 我觉得缺乏组织研究的机构资源 肿瘤表型核心将大大加强研究 在医学院的能力。这一核心将与两个 其他提出的核心，提供（1）基因组学/蛋白质组学和（2） 常规组织学、组织微阵列制备和激光捕获 显微解剖肿瘤表型核心将为以下人员提供资源 自动免疫组织化学，原位杂交，荧光原位 杂交（FISH）、RNA和DNA分离、荧光显微镜和真实的 时间PCR。这一核心将为目前受资助的30个国家癌症研究所提供资源。 研究人员在该机构，以及提供种子赠款的钱， 启动项目，以鼓励更多的癌症相关研究， 机构。资源将根据秘书长对提议的审查加以利用。 内部咨询委员会，并将提供人员以及50%的 核准项目的供应费用。这个核心是一个电流的扩展 病理学系中为临床和研究服务的设施 机构的需要。扩充人员和供应 美元将使这一核心服务于许多研究人员在癌症 社区

DESCRIPTION (provided by applicant): In 1996, Ohio's death rate from all cancers was 13th highest among the 50 states and the District of Columbia. Ohio ranked 16th highest for death rates from lung cancer, 12th from colorectal cancer and 6th for breast cancer deaths. The area in and around Cincinnati is in the top 10th percentile of mortality from cancer for Blacks, Whites, males and females. Hamilton County (site of the University of Cincinnati) has a statistically significant higher cancer incidence than Ohio overall. These data have ignited clinicians, researchers, and administrators to develop collaborative programs that will spark new investigations into the basic biology of cancer and foster programs to allow translation of this new knowledge into clinical care. This proposal is one of four that are the direct result of a series of discussion groups, including clinicians and basic researchers, to develop program strategies to meet these clinical needs. Both clinical/translational and basic researchers felt there was a paucity of institutional resources for tissue-based research and that a Tumor Phenotyping Core would significantly enhance the research capacity in the College of Medicine. This core will work closely with two of the other proposed cores, which provide (1) genomics/proteomics and (2) routine histology, preparation of tissue microarrays, and laser capture microdissection. The Tumor Phenotyping Core will provide resources for automated immunohistochemistry, in situ hybridization, fluorescence in situ hybridization (FISH), RNA and DNA isolation, fluorescence microscopy, and real time PCR. This core will provide resources to the 30 currently NCI funded investigators in the institution, as well as providing seed grant monies for start up projects to encourage additional cancer related research in the institution. Resources will be utilized based upon review of proposals by an Internal Advisory Committee and will provide personnel as well as 50% of supply costs for approved projects. This core is an expansion of a current facility in the Department of Pathology that serves clinical and research needs in the institution. Expansion of personnel and availability of supply dollars will allow this core to serve numerous investigators in the cancer community.