UCLA Imaging Resource for Mouse Cancer Models

描述(申请人提供)：加州大学洛杉矶分校拥有成熟的小动物影像 基于数字全身放射自显影(DWBA)和微正电子的程序 发射断层扫描(MicroPET)。我们在这项研究上投入了大量资金 利用PET报告基因技术建立小动物癌症模型。记者 基因与DWBA和microPET相结合，为我们提供了 研究癌症生物学、细胞转运和基因的临床前模型 心理治疗。正是通过这些研究，我们更好地理解了 我们现有技术的局限性，因此提出收购建议 微型计算机断层扫描(MicroCAT)和光学电荷耦合器件 (电荷耦合器件)成像系统。我们还将购买一台急需的计算机 用于产生大量数据的服务器和数据归档系统 通过这项工作。我们最初将允许六名经验丰富的癌症调查人员 使用资源，然后通过向每个调查员添加最多三个调查员来实现增长 年。 MicroCAT将允许我们对小鼠癌症的潜在解剖结构进行成像 模特们。这将有助于我们了解(S)的位置 各种细胞活动，而不需要牺牲动物。更有甚者 通过这项工作中提出的直接研究，我们将注册microPET 和microCAT信息，为我们的研究人员提供最大的信息 在功能和解剖学上。光学ccd系统也将在 帮助加速我们的癌症相关研究。萤火虫的用途 荧光素酶(Flc)报告基因将使我们能够更快地研究小 没有放射性探头的动物模型。该系统具有以下功能： 从活着的小动物体内成像低水平的光。我们将使用 将Flc和PET报告基因连接在一起的报告系统 无论是在光学CCD系统中还是在微型PET中都可以灵活地成像。这 将有助于加快发展各种依赖于 关于记者基因技术。对所有医疗设备的数据进行量化将 在SAIRP的整个过程中始终要强调。 我们还将制定一个强有力的培训计划，帮助调查人员 和他们的学生变得独立和自信地使用可用的 资源。整个资源的使用将由基于互联网的 调度软件和一个监督委员会。我们相信，新的 使用微CAT和光学CCD技术的资源将有助于提供加州大学洛杉矶分校 研究人员使用最先进的工具对小鼠癌症进行非侵入性成像 模特们。

DESCRIPTION (provided by applicant): UCLA has a mature small animal imaging program based on digital whole body autoradiography (DWBA) and micro positron emission tomography (microPET). We have significant investments in the study of small animal cancer models using PET reporter gene technology. Reporter genes in combination with DWBA and microPET have provided us the ability to study cancer biology, cell trafficking, and pre-clinical models for gene therapy. It is through these studies that we have better understood the limitations of our current technologies and therefore propose the acquisition of micro computed tomography (microCAT) and optical charge coupled device (CCD) imaging systems. We will also acquire a critically needed computer server and data archiving system for the large amounts of data generated through this work. We will initially allow six seasoned cancer investigators to use the resource and then grow by adding up to three investigators per year. MicroCAT will allow us to image the underlying anatomy in our mouse cancer models. This will be critical in helping us to understand the location(s) of various cellular events without the need to sacrifice the animals. Furthermore through direct research proposed in this work we will register the microPET and microCAT information to provide our researchers with maximal information on function and anatomy. The optical CCD system will also be critical in helping to accelerate our cancer related research. The use of the firefly luciferase (FLUC) reporter gene will allow us to more rapidly study small animal models without radioactive probes. This system has the capability of imaging low levels of light from within a living small animal. We will use reporter systems that couple FLUC and PET reporter genes in order to have the flexibility to image in either an optical CCD system or the microPET. This will help to accelerate the development of various models that are dependent on reporter gene technology. Quantitation of data from all modalities will always be stressed throughout the SAIRP. We will also develop a strong training program that will help investigators and their students to become independent and confident in using the available resources. Use of the entire resource will be coordinated by intemet based scheduling software and an oversight committee. We are confident that the new resources with microCAT and optical CCD technologies will help to provide UCLA investigators with state-of-art tools for non-invasively imaging mouse cancer models.