Myogenic Stem Cell Function in Aging Skeletal Muscle

超出所提供的空间。 骨骼肌的质量、力量和再生能力随着年龄的增长而下降。增龄肌肉中肌源性前体细胞增殖的增强可以提高肌纤维修复的效率，从而减少与年龄相关的肌肉萎缩。因此，鉴定能够增强衰老肌肉中生肌前体功能的生理试剂具有重要意义。卫星细胞（位于肌纤维表面）被公认为是生后肌肉中的成肌前体细胞。虽然卫星细胞通常作为组织特异性肌源性干细胞发挥作用，但最近已从成人肌肉中分离出能够产生肌源性细胞的多能干细胞。深入了解成人和衰老骨骼肌中生肌前体的起源以及源自这些前体的细胞类型可以进一步帮助设计治疗方法，以改善衰老肌肉中生肌前体的数量和功能。 本研究的目的是：1）研究成纤维细胞生长因子2（FGF 2）对衰老肌肉卫星细胞增殖的影响。2)研究成年和衰老啮齿类动物血管来源的细胞产生骨骼肌前体的潜力。3)检测血管来源的细胞参与体内肌纤维修复的能力。在整个研究过程中将使用小鼠。将在分离的肌纤维培养物中分析FGF对卫星细胞增殖的影响。将使用骨骼肌和非骨骼肌来源的血管检查血管系统产生骨骼肌源性细胞的能力。将通过免疫细胞化学和RT-PCR在克隆培养物中表征血管来源的细胞。体内研究将检查来自脉管系统的细胞在肌营养不良蛋白缺陷mdx裸鼠的宿主肌肉中贡献肌纤维核和卫星细胞的能力。 拟议的调查将有助于新的洞察肌源性前体在成人和衰老肌肉的状态，并可以证明在疾病和衰老过程中的肌肉康复策略的重要性。性能现场=

EXCEED THE SPACE PROVIDED. Skeletal muscle mass, strength and regenerative capacity decline with aging. Enhanced proliferation of myogenic precursor cells in aging muscle could improve the efficiency of myofiber repair and lead to a reduction in age-linked muscle atrophy. Thus, the identification of physiological agents capable of enhancing the function of myogenic precursors in the aging muscle is of significant importance. Satellite cells (located on the surface of the myofiber) are well recognized as the myogenic precursors in postnatal muscle. While satellite cells typically function as tissue-specific myogenic stem cells, multipotent stem cells, capable of giving rise to myogenic cells, have been recently isolated from adult muscle. Gaining insight into the origins of myogenic precursors in adult and aging skeletal muscle as well as the types of cells derived from these precursors can further assist in the design of therapies to improve the number and function of myogenic precursors in aging muscle. The aims of this proposal are: 1) To investigate the effect of fibroblast growth factor (FGF2) on the proliferation of satellite cells in aging muscle. 2) To investigate the potential of cells derived from the vasculature of adult and aging rodents to give rise to skeletal myogenic precursors. 3) To examine the capacity of cells derived from the vasculature to participate in myofiber repair in vivo. Mice will be used throughout the investigations. The effect of FGF on the proliferation of satellite cells will be analyzed in cultures of isolated myofibers. The capacity of the vasculature to give rise to skeletal myogenic cells will be examined with vessels from skeletal muscle and non-skeletal muscle origins. The vasculature-derived cells will be characterized in clonal cultures by immunocytochemistry and RT-PCR. The in vivo studies will examine the ability of cells derived from the vasculature to contribute myofiber nuclei and satellite cells in host muscle of dystrophin-deficient mdx nude mice. The proposed investigation will contribute new insight into the status of myogenic precursors in the adult and aging muscle and can prove important for muscle rehabilitation strategies during disease and aging. PERFORMANCE SITE ========================================Section End===========================================