Chemical Regulation of Zebrafish Gene Expression

虽然斑马鱼已经成为脊椎动物生物学的理想模型，但用于评估斑马鱼的方法仍然存在不足。 斑马鱼的基因功能仍然不发达。这个应用程序描述了两个跨学科的战略 用于通过化学试剂在时间和空间上控制斑马鱼中的基因表达。一种方法 建立在斑马鱼群体使用吗啉代低聚物选择性抑制基因表达的基础上 并且涉及笼状吗啉代的合成，所述笼状吗啉代在被光激活时变得发荧光。的 另一种方法使用蜕皮激素受体激动剂诱导转录激活， 提供额外空间控制的激动剂。这两种策略都将产生具有定制基因的斑马鱼 表达模式，为在分子水平上理解脊椎动物生理学提供了新的机会。 系统层面。由于斑马鱼被用于研究脊椎动物生物学的多个方面，这些新的 实验能力将促进我们对人类发育、生理和疾病的理解。

Although the zebrafish has emerged as an ideal model of vertebrate biology, methods for evaluating zebrafish gene function remain underdeveloped. This application describes two interdisciplinary strategies for temporally and spatially controlling gene expression in zebrafish through chemical reagents. One method builds upon the zebrafish community's use of morpholino oligomers to selectively inhibit gene expression and involves the synthesis of caged morpholinos that become fluorescent upon their activation by light. The other approach uses ecdysone receptor agonists to induce transcriptional activation, with photoactivatable agonists providing additional spatial control. Both strategies will generate zebrafish with tailored gene expression patterns, providing new opportunities for understanding vertebrate physiology at the molecular and systems levels. Since zebrafish are used to study multiple aspects of vertebrate biology, these new experimental capabilities will advance our understanding of human development, physiology, and disease.