DNA SEQUENCING AND ANALYSIS CORE FACILITY

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 DNA分析，特别是大量DNA基因组信息的应用，是现代生物医学研究的一个重要方面。 为了促进应用最新技术研究与得克萨斯/墨西哥边境地区有关的问题，本提案将支持开发一个DNA分析核心设施。 该设施将把联合收割机现有的共享设备和研究者拥有的设备（包括DNA测序、基因型分析、真实的时间PCR和专业电泳能力）合并为一个管理良好的、研究者可访问的核心设施。 除了提供工作人员支持外，该提案还将通过增加一个定制的微阵列设施来增强现有的仪器基础设施。 这种新的定制阵列能力是支持毒物应激、感染性疾病的分子流行病学和代谢紊乱的现有研究项目发展的一个基本要素。 虽然该设施最初将在现有空间内建立，但在这些新实验室完工后，它将搬到位于埃尔帕索的德克萨斯大学新生物医学科学大楼的扩建区。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. DNA analysis, particularly the application of the deluge of DNA genomic information, is an essential aspect of modern day biomedical research. In order to facilitate the application of state of the art techniques to research problems relevant to the Texas/Mexico border region, this proposal will support the development of a DNA analysis core facility. This facility will combine existing shared and investigator owned equipment, including DNA sequencing, Genotype analysis, real time PCR, and specialized electrophoresis capability into a managed, researcher accessible core facility. In addition to providing staff support, this proposal will augment existing instrument infrastructure by the addition of a custom microarraying facility. This new custom arraying capability is an essential element in support of development of existing research projects in toxicant stress, the molecular epidemiology of infectious diseases, and metabolic disorders. While the facility will initially be setup in existing space, it will move to expanded quarters in the new Biomedical Sciences Building at the University of Texas in El Paso upon completion of those new laboratories.