INFRASTRUCTURE 1: CORE RESEARCH LABORATORIES

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 塔斯基吉大学CBR/RCMI核心设施通过为研究人员提供使用显微镜/成像、流式细胞术、细胞培养和分子生物学以及生物信息学和计算生物学所需的仪器、设施和技术援助，促进了多学科研究。核心设施向代表3所学术学院和9个科学学科的用户提供了服务和援助。这些调查人员在2007-2008年预算期间发表或提交了十(10)篇同行评议的论文，并提交了八(8)篇论文或海报。成像设备被最多的研究人员使用。通过将徕卡DM 5000B(直立式显微镜)与现有的Q IMAGING CE数码相机(通过C IMAGING Systems Simple PCI软件操作)相结合，大大增强了荧光显微镜的性能。该系统允许对标记有多个荧光探针的标本的图像进行可视化和数字捕获，并将多个图像融合成一张照片。此外，还获得了配备细胞孵化室的徕卡DM IRE2(倒置荧光显微镜)。该系统还配备了类似的数字摄影系统，将允许在更长的时间段内可视化活细胞实验。细胞培养和分子生物学实验室为卡弗研究实验室的研究人员(和他们的学生)提供现场设施，积累了最多的“工时”。生物信息学和计算生物学基金通过使用基于网络的基础设施，特别是基因组学和蛋白质组学，努力增加对生物信息学和计算生物学研究的参与。为此，生物系开设了两门生物信息学课程，生物学0368(本科生)和0568(研究生)。总而言之，在对CBR/RCMI核心I设施的5年支持期间，除了对细胞培养和分子生物学设施(孵化器、离心机、冷冻机等)的正常维护外，还增加了几种提供更复杂技术的新仪器。这些设备包括：Bio-TEK Synergy HT微板阅读器、Eppendorf Mastercler、Cepheid SmartCycle 2以及上述显微镜。核心综合设施向至少24名调查人员及其学生提供了服务，他们代表了广泛的科学研究领域。这些研究人员发表或提交了十七(17)篇同行评议的论文，并提交了三十五(35)篇论文或海报。总体而言，核心i项目显着提高了塔斯基吉大学研究人员的研究能力和生产力。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Tuskegee University CBR/RCMI Core Facility facilitates multidisciplinary research by providing investigators with the instrumentation, facilities and technical assistance necessary to utilize Microscopy/Imaging, Flow Cytometry, Cell Culture and Molecular Biology, and Bioinformatics and Computational Biology. The Core Facility has provided service and assistance to users representing 3 academic colleges and 9 scientific disciplines. These investigators published or submitted ten (10) peer reviewed papers and presented eight (8) papers or posters in the 2007-2008 budget period. The Imaging Facility is used by the most researchers. Fluorescence microscopy, has been greatly enhanced by the addition of a Leica DM 5000B (upright microscope) coupled to the existing Q Imaging CE digital camera (operating through C Imaging Systems Simple PCI software). This system allows the visualization and digital capturing of images of specimens tagged with multiple fluorescent probes and the fusion of the multiple images into a single photograph. Additionally, a Leica DM IRE2 (inverted fluorescent microscope) equipped with a cell incubation chamber has been acquired. This system is also equipped with a similar digital photography system and will allow the visualization of live cell experiments over extended time periods. The Cell Culture and Molecular Biology laboratories accumulate the most "man hours" of use as they provide on site facilities for the investigators (and their students) working in Carver Research Laboratories. The Bioinformatics and Computational Biology Facility strives to increase participation in bioinformatics and computational biology research through the use of web based infrastructure, especially in genomics and proteomics. Toward this end, the Department of Biology has initiated two courses in Bioinformatics, Biology 0368 (undergraduate) and 0568 (graduate). In summary, during the 5 years of support for the CBR/RCMI CORE I Facility, in addition to normal maintenance of the cell culture and molecular biology facilities (incubators, centrifuges, freezers, etc.), several new instruments providing more sophisticated technologies have been added. These include: BIO-TEK SYNERGY HT microplate reader, Eppendorf Mastercycler, Cepheid Smartcycler 2, and the microscopes described above. The CORE I Facility has provided services to at least 24 investigators and their students representing a broad spectrum of scientific research. These investigators published or submitted seventeen (17) peer reviewed papers and presented thirty-five (35) papers or posters. On the whole the CORE I project has significantly enhanced the research capabilities and productivity of the Tuskegee University research faculty.