ELECTRON MICROSCOPY CORE

电子显微镜(EM)核心是NIDA赞助的成瘾中心的关键组件，该中心 提供有助于发射机的蜂窝和亚蜂窝本地化的资源和服务， 受体和转运体正在调查中。这些对中国的所有基础科学研究都很重要 项目1-3，对项目3中的定量电子显微镜免疫标记至关重要。核心 人员包括威尔医学院神经病学和神经科学系的两名教职员工。 康奈尔大学学院。主任皮克尔博士开创并确立了植入前的许多方面 对中枢神经系统中的各种抗原进行EM免疫标记，并具有 在脑解剖学和光镜下检测信使核糖核酸和蛋白质方面具有丰富的专业知识 项目1和2。联席主任格拉斯博士在使用量化的 成瘾物质影响下受体转运的形态计量学分析。资源 核心设备包括CM-10和TECI电子显微镜以及所有较小的设备 用于LM和EM结构分析的种子。核心将提供与(A)有关的服务和设施 LM和EM免疫标记抗血清的鉴定及组织的处理 (B)原位杂交 (C)图像采集和免疫标记的量化。《核心》 教职员工和资源位于街对面的康奈尔大学威尔医学院 来自洛克菲勒大学，占据了基普斯湾大楼的一层。进入这个设施很容易 由洛克菲勒大学获得，与提交适当的身份证明有关。 新兴市场资源的目标是： 1.提供最先进的特定脑区组织的超微结构检查，利用标准 和新形式的电子显微镜和中心的其他标准成像技术，包括 项目3，可能是项目1和2，以及任何需要这项技术的试点项目。 2.根据需要开发新的超微结构成像技术。 3.对科学家和技术支持人员进行各种电子显微镜和相关技术的培训 成像技术。

The Electron Microscopy (EM) Core is a critical component of the NIDA sponsored addiction center, which provides resources and services contributing to the cellular and subcellular localization of transmitters, receptors and transporters under investigation. These are important for all the basic science research in Projects 1-3, and crucial for the quantitative electron microscopic immunolabeling in Project 3. The core personnel include two faculty members of the Department of Neurology and Neuroscience, Weill Medical College of Cornell University. The Director, Dr. Pickel, has pioneered and established many facets of preembedding EM immunolabeling of a variety of antigens in the central nervous system, and also has substantial expertise in brain anatomy and LM detection of mRNA and proteins under investigation in Projects 1 and 2. The co-director, Dr. Glass has made significant contributions to the use of quantitative morphometric analysis of receptor trafficking under the influence of addictive substances. The resources available in the core include a CM-10 and Techni electron microscopes as well as all smaller equipment seeded for LM and EM structural analysis. The core will provide services and facilities related to (a) characterization of antisera and processing of the tissue for both LM and EM immunolabeling either before (pre-) or after (post-) embedding in resin to for microscopic analysis, (b) in situ hybridization of mRNA on frozen slide-mounted brain sections, (c) image acquisition and quantification of immunolabeling. The Core faculty and resources are located at Weill Medical College of Cornell University, which is across the street from Rockefeller University, and occupies one floor of the Kips Bay building. Access to this facility is easily gained by Rockefeller University associates with the presentation of proper identification. The Goals of the EM Resourse are: 1. To offer state of the art ultrastructural examination of tissues from specific brain region, utilizing standard and novel forms of electron microscopy and other standard imaging techniques, for the Center, including Project 3, possibly Projects 1 and 2, and any pilot projects which require this technology. 2. To develop new technologies for ultrastructural imaging as needed. 3. To train scientists and technical support staff in the various techniques of electron microscopy and related imaging techniques.