Histology and Imaging Core

该中心内的项目研究皮肤溃疡病毒(ECTV)与病原体之间的相互作用 鼠痘(老鼠的天花)及其自然宿主--老鼠。ECTV之间的相互作用 宿主的免疫系统可以在许多不同的水平上发生。它可以在组织水平上发生， I型干扰素等免疫介质的释放改变皮肤感染(项目1)或 淋巴结、脾和肝脏(方案2)由ECTV.，它也可以发生在细胞水平，在其中免疫 细胞渗透和与ECTV感染细胞的相互作用将改变病毒的复制和传播(项目1，3)。 最后，它可以发生在细胞内的水平上，在这种水平上，内化的ECTV的运输和内吞 在ECTV感染期间使用的途径，可以改变免疫系统感知的机制 感染(本核心的项目3+目标2)。这个核心(C)将允许可视化所有这些事件，通过 为所有三个项目提供及时、经济、专业的组织学和显微镜支持 中心。此核心将提供的设备和专业知识旨在减少启动 个人用户在需要设计时所需的时间和专业知识程度 并进行实验和图像处理。此外，核心将允许成像活病毒在 生物安全2级遏制，这在共享设施中是不可能的。除了提供以下支持之外 这三个项目，这个核心的目标2将调查一个研究领域，连接调查领域 在项目1和3中，即ECTV内化和感染所涉及的内吞过程，以及 病毒衍生成分的呈现与MHC II类复合体。因此，核心将增强 该中心的科学能力和将促进各个项目之间的互动。

The Projects within this Center study the interplay between Ectromelia virus (ECTV), the causative agent of mousepox (the smallpox of the mouse), and its natural host, the mouse. The interplay between ECTV and the immune system of the host can take place at many different levels. It can occur at a tissue level, in which release of immune mediators such as type I interferons alter infection in the skin (Project 1) or lymph node, spleen and liver (Project 2) by ECTV., It can also occur at the cellular level, in which immune cell infiltration and interaction with ECTV infected cells will alter virus replication and spread (Project 1, 3). Finally, it can occur at an intracellular level, in which the trafficking of internalized ECTV, and the endocytic routes used during ECTV infection, can alter the mechanisms by which the immune system senses infection (Project 3 + Aim 2 of this Core). This Core (C) will allow the visualization of all of these events, by providing timely, cost-effective and expert histology and microscopy support for all three Projects within the Center. The equipment and expertise that will be provided by this Core are aimed at reducing the startup time and degree of expertise that would be necessary for individual users if they were required to design and execute experiments and image processing. In addition, the Core will allow imaging of live virus in Biosafety Level 2 containment, which is not possible in shared facilities. In addition to providing support for all three Projects, Aim 2 of this Core will investigate an area of research that bridges areas of investigation in Projects 1 and 3, namely the endocytic processes involved in ECTV internalization and infection, and the presentation of virus-derived components in complex with MHC Class II. Thus, the Core will enhance both the scientific capabilities of the Center and will foster interactions between the individual Projects.