Protein Expression Core

蛋白质表达核心将利用沃兹沃斯中心在以下领域的显著优势 分子遗传学和蛋白质生物化学。蛋白质生产核心的目标将是促进 在本申请的不同主题中提出的调查性、发展性和先导性研究。的工作人员 核心将协助研究人员将他们的研究转化为质量控制的标准分析， 疫苗或疗法。蛋白质表达核心的工作人员将与调查人员合作开发 使用商业载体或定制的研究所需的抗原的表达系统 表达载体。该设施将提供完整的蛋白质表达和纯化服务。工作人员 的蛋白质生产核心将生产高度纯化的标准化的大量重组蛋白质抗原， 重组蛋白质或蛋白质片段、纯化的抗体和修饰的蛋白质 多重诊断分析和新型疫苗开发的研究人员。在疫苗的情况下 开发后，Core将提供细菌成分污染较低的蛋白质，适用于 动物研究。该核心旨在为科学家提供咨询和实践支持，这些科学家需要 大量纯化的重组蛋白用于他们的研究。核心的一个目标将是准备 稳定的蛋白质和表达菌株的储备，以便在紧急情况下生产这些材料 将是快速的。

The Protein Expression Core will draw on the significant strengths of the Wadsworth Center in the areas of molecular genetics and protein biochemistry. The goal of the Protein Production Core will be to facilitate the investigative, developmental, and pilot studies proposed in various themes of this application. The staff of the Core will assist investigators in the translation of their studies to quality controlled standard assays, vaccines or therapeutics. Staff of the Protein Expression Core will work with investigators to develop expression systems for the antigens required for their studies either using commercial vectors or customized expression vectors. The facility will provide a complete protein expression and purification service. The staff of the Protein Production Core will produce highly purified standardized lots of recombinant protein antigens, recombinant proteins or protein fragments, purified antibodies and modified proteins as needed by the investigators for the development of multiplex diagnostic assays and novel vaccines. In the case of vaccine development, the Core will provide proteins with low bacterial component contamination suitable for use in animal studies. The Core is designed to provide both advisory and hands on support to scientists, who need larger amounts of purified recombinant proteins for their investigations. A goal of the Core will be to prepare stable stocks of proteins and expression strains so that in a time of emergency production of these materials will be rapid.