High-throughput Screening Core

高通量筛选(HTS)核心实验室(核心B)的目的是创建和实施HTS 筛选小分子，以确定那些可以成为开发癌症疗法或 癌症研究中的工具。此外，HTS实验室进行基因组RNAi筛选以识别新的 开发癌症治疗药物的目标。 核心B管理UTSW化合物档案，目前包括20多万个小类药物 有机化合物。核心B开发PPG项目的分析、筛选和分析 筛选数据。HTS实验室进行验证感兴趣化合物所需的二次筛选 在初步筛选实验中鉴定出来的。与PPG的调查人员合作，科学家们在 核心B和化学核心将高温超导实验中确定的感兴趣的化合物和 管理这些化合物的再补给和储存。HTS核心维护一个Oracle数据库 筛选数据，以便可以将任何HTS实验中感兴趣的化合物的活性与所有 使用UTSW复合文件进行的其他HTS实验。HTS Core还管理和 为PPG项目进行人类基因组siRNA文库的HTS筛选实验。 除了为NCI资助的PPG成员进行HTS筛查外，Core B还培训和建议 德克萨斯大学西南分校综合癌症中心成员谈发展和实施 小分子和基因组siRNA HTS实验，并将此类实验的数据存储为 UTSW筛查数据库。 HTS设施通过为癌症研究人员提供数百个 数千种结构各异的化合物，可用于癌症的药物治疗或工具 研究是可以确定的。

The purpose of the high-throughput screening (HTS) core laboratory (Core B) is to create and conduct HTS screens of small molecules to identify those that can become leads for developing cancer therapeutics or tools in cancer research. In addition, the HTS laboratory conducts genomic RNAi screens to identify novel targets for developing cancer therapeutics. Core B manages the UTSW chemical compound file currently including more than 200,000 small drug-like organic compounds. Core B develops assays for the projects of the PPG,screens them and analyzes screening data. The HTS lab conducts secondary screens required to validate compounds of interest identified during primary screening experiments. Working with the investigators of the PPG, scientists in Core B and the Chemistry Core prioritize the compounds of interest identified in HTS experiments and manage resupply and storage of those compounds. The HTS core maintains an Oracle database of screening data so that the activity of compounds of interest in any HTS experiment can be compared to all other HTS experiments conducted with the UTSW compound file. The HTS Core also manages and conducts HTS screening experiments of a human genomic siRNA library for the Projects of the PPG. In addition to conducting HTS screens for members of this NCI funded PPG, Core B trains and advises members of the Comprehensive Cancer Center at UT Southwestern on the development and execution of small molecule and genomic siRNA HTS experiments and stores the data from such experiments as part of the UTSW screening database. The HTS facility advances cancer research by providing cancer researchers access to hundreds of thousands of structurally diverse compounds from which leads for drug therapeutics or tools for cancer research can be identified.