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The Knockout of the Mouse Muc5ac

The Knockout of the Mouse Muc5ac
小鼠 Muc5ac 的敲除
批准号：
7594029
负责人：
Robert Langenbach
金额：
$45.76万
依托单位：
NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES
依托单位国家：
美国
项目类别：
财政年份：
--
资助国家：
美国
项目状态：
未结题
起止时间：
至
来源：
https://reporter.nih.gov/project-details/7594029
关键词：
CellsChimera organismGastric ulcerGenesGenomicsHumanInfectionInflammationKnockout MiceLungMolecularMucinsMusNeomycinPTGS2 genePathway interactionsPharmacologyPhysiologicalPolymerase Chain ReactionPredispositionReportingRoleStomachblastocystembryonic stem cellinsightmalignant stomach neoplasmtransmission process

项目摘要

项目成果

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相关文献

中文摘要
翻译
我们构建了一个包含新霉素和HSTK基因以及10kb基因组同源性的靶向构建体来破坏Muc5ac基因。该结构已被电穿孔到小鼠胚胎干细胞中，但迄今为止，仅获得了约1000个PCR阳性克隆。这些克隆正在通过PCR和基因组Southern分析进一步分析，以验证靶向性。一旦确定靶向性，这些克隆将被注射到小鼠囊胚中产生嵌合体并实现种系传播。
英文摘要
A targeting construct containing the neomycin and HSTK genes and 10 kb of genomic homology has been constructed to disrupt the Muc5ac gene. The construct has been electroporated into mouse ES cells but to date only 2 PCR positive clones of about 1,000 analyzed have been obtained. These clones are being further analyzed by PCR and genomic Southern analysis to verify targeting. Once targeting is confirmed these clones will be injected into mouse blastocyst to generate chimeras and achieve germline transmission.
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