Ultra Fast and Sensitive Fluorescence-Based Clinical Assays

描述（由申请人提供）： Kadir Asian博士是一位具有工程背景的研究人员，他有很大的潜力可以将职业过渡到独立的生物医学研究人员。Asian博士在纳米技术方面拥有丰富的研究经验，他在基于金属纳米颗粒的生物传感平台的新制备技术及其随后在蛋白质和DMA检测生物测定中的应用领域的文献中做出了贡献。然而，Asian博士在光谱学和生物化学方面的知识和经验有限，在细胞培养和分子生物学技术方面没有经验，而这些技术是生物医学研究人员必不可少的工具。拟议的综合发展局会透过高级生物学课程、部门研讨会、专业学会会议和分子生物学实验室技术工作坊，向他介绍生物学。作为拟议研究的结果，亚洲博士将获得先进的生物化学和分子生物学知识以及相应的实验室技术，以确定和提出生物医学问题的解决方案，并执行和指导相关研究。此外，Asian博士还将接受Chris D. Geddes是世界著名的荧光光谱学专家，并由Joseph P.Y.博士指导生物技术。他是纳米和细胞生物学专家。拟议的光谱学和生物学的指导培训与他的纳米技术背景相结合，将迎来亚洲博士在生物医学研究的独立职业生涯。拟议的研究计划包括：通过课程和研讨会（1/4努力）密集学习光谱学，生物学和生物实验室技术的一年;新的生物传感平台的开发（与他过去的研究一致，1/4努力）;两个临床相关测定的开发（2/4努力）1）临床环境的心脏标志物测定，和2）p53蛋白测定。心血管疾病和癌症是发达国家的主要死亡原因。在这项拟议的研究中，将开发新的检测方法，可以在非常早期的阶段检测相关的指示蛋白，以评估这些疾病，用于临床环境中的潜在下游用途。

DESCRIPTION (provided by applicant): This Career Development Award application is for Dr. Kadir Asian, a researcher with an engineering background, who has great potential to make a career transition into an independent biomedical researcher. Dr. Asian has extensive research experience in nanotechnology, where he made contributions to the literature in the field of new preparation techniques for metal nanoparticle-based biosensing platforms and their subsequent applications in bioassays for protein and DMA detection. However, Dr. Asian has limited knowledge and experience in spectroscopy and biochemistry and no experience in cell culture and molecular biology techniques, which are essential tools for a biomedical researcher. The proposed CDA will introduce him to biology through advanced biology courses, departmental seminars, professional society meetings and workshops on molecular biology laboratory techniques. As a result of the proposed research, Dr. Asian will gain advanced biochemistry and molecular biology knowledge as well as corresponding laboratory techniques to identify and propose solutions to biomedical problems and perform and direct related research. In addition, Dr. Asian will also be trained in advanced spectroscopic techniques by Dr. Chris D. Geddes, a world renowned expert in fluorescence spectroscopy, and mentored in biological techniques by Dr. Joseph P.Y. Kao, an expert in nano- and cell biology. Proposed mentored training in spectroscopy and biology combined with his nanotechnology background will usher Dr. Asian into an independent career in biomedical research. The proposed research plan includes: a year of intense learning of spectroscopy, biology and biology laboratory techniques through courses and workshops (1/4th effort); the development of new biosensing platforms (consistent with his past research, a 1/4th effort); the development of two clinically relevant assays (2/4th effort) 1) cardiac marker assays for a clinical setting, and 2) p53 protein assays. Cardiovascular diseases and cancer are amongst the leading causes of mortality in developed countries. In this proposed research, new assays that can detect the relevant indicator proteins for the assessment of these diseases at very early stage will be developed for the potential downstream use in a clinical setting.