UMBRELLA UMBILICAL CORD PROTOCOL FOR ENDOTHELIAL CELL ISOLATION

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 本研究的目的是研究真菌烟曲霉与内皮细胞的相互作用。 我们的假设是A.在侵袭性曲霉病的发病机制中，烟曲霉菌与内皮细胞在血管侵袭过程中的相互作用是重要的。对于拟定的研究，将从人脐带内的静脉中收获内皮细胞，并在组织培养物中生长。 Drs.加州大学洛杉矶分校哈伯医学中心洛杉矶生物医学研究所的约翰·爱德华兹、斯科特·福勒和他的同事们已经请求西达斯-西奈综合临床研究中心协助收集脐带。脐带在正常分娩后会被丢弃，但它可以用来分离内皮细胞（血管内部的细胞）。然后，这些细胞可以用于各种旨在了解真菌感染基础生物学的研究项目。Harbor-UCLA的交付数量不足以提供他们研究所需的内皮细胞数量，而Cedars-Sinai的大量交付为这些收集提供了理想的位置。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The purpose of this research project is to study the interactions of the fungus, Aspergillus Fumigatus, with endothelial cells. Our hypothesis is that the interactions of A. Fumigatus with endothelial cells during the process of angioninvasion are central to the pathogenesis of invasive Aspergillosis. For the proposed studies, the endothelial cells will be harvested from the veins within human umbilical cords and grown in tissue culture. Drs. John Edwards, Scott Filler and colleagues at the Los Angeles Biomedical Research Institute at Harbor-UCLA Medical Center have requested assistance from the General Clinical Research Center at Cedars-Sinai in collecting umbilical cords. Umbilical cords, that would otherwise be discarded following normal deliveries, can be used to isolate endothelial cells (cells that line the inside of blood vessels). These cells can then be used in a variety of research projects aimed at understanding the basic biology of fungal infections. The number of deliveries occurring at Harbor-UCLA is not sufficient to provide the numbers of endothelial cells required for their research, while the very large volume of deliveries at Cedars-Sinai offers an ideal location for these collections.