SALINE/DIURETIC STUDY

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 我们提出了一项研究，目的是为钠潴留的遗传研究产生表型，钠潴留是一种易患高血压的疾病。 我们专注于三个肾单位区域，厚升支钠，钾，2氯共转运蛋白运作，钠-氯共转运蛋白在远曲小管，和上皮钠通道在集合管。 将有200名年龄在18-35岁之间的无关受试者参加，黑人和白人、男性和女性的人数相等。 受试者将在研究前一天下午入住综合临床研究中心，在那里他们将接受高钠饮食。 第二天早上，将在3小时内输注1升生理盐水。 我们将评估受试者如何排泄（或保持）钠负荷（观察时间超过4小时）。 然后，受试者将在30分钟内静脉注射10 mg“袢利尿剂”呋塞米，然后观察4小时。 父母一方或双方将提供DNA血样，以便进行额外的传播不平衡基因检测。 这将是一个重要的协议开发表型的钠潴留，和敏感性的血压钠。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We propose a study for the purpose of generating phenotypes for genetic studies of sodium retention, a condition that predisposes to high blood pressure. We focus on three nephron regions, the thick ascending limb where the sodium, potassium, 2 chloride co-transporter operates, the sodium-chloride co-transporter in distal convoluted tubule, and the epithelial sodium channel in the collecting duct. Two hundred unrelated subjects, equal numbers of blacks and whites, males and females, ages 18-35 years, will participate. Subjects will be admitted to the General Clinical Research Center the afternoon before study where they will receive a diet high in sodium. The following morning, one liter of normal saline will be infused over 3 hours. We will assess how subjects excrete (or hold onto) the sodium load (observations will be made over 4 hours). Then subjects will be given a "loop-diuretic," furosemide, 10 mg intravenously over 30 minutes, and then observed over a period of 4 hours. One or both parents will give a blood sample for DNA to allow for performing the additional genetic testing of transmission disequilibrium. THis will be an important protocol for developing the phenotypes of sodium retention, and sensitivity of the blood pressure to sodium.