REGULATION OF LYMPHOCYTE AND MONOCYTE IMMUNE FUNCTIONS BY VITAMIN A COMPOUNDS

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 目的是测试人血单核细胞和淋巴细胞在暴露于维生素A或相关类维生素A培养物后是否显示抗原呈递细胞（APC）的特征。抗原呈递对于免疫应答至关重要，包括CD 4和CD 8 T细胞活化。该假设是基于我们对转化的人单核细胞系THP-1进行的研究，该研究表明，暴露于生理浓度的视黄酸（维生素A的活性代谢物）诱导参与抗原呈递的分子的表达：CD 1d，一种MHC-I样分子（涉及脂质抗原呈递）、MHC-II分子（肽抗原呈递）和DC-SIGN（CD 209，碳水化合物抗原呈递）。这些数据表明，视黄酸诱导THP-1单核细胞成为APC。为了证明RA的这种作用不仅限于转化细胞，重要的是要证明正常人单核细胞可以类似地调节。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The purpose is to test whether human blood monocytes and lymphocytes display characteristics of antigen-presenting cells (APCs) after exposure in culture to vitamin A or related retinoids. Antigen presentation is critical for immune responses, including CD4 and CD8 T cell activation. The hypothesis is based on work we have conducted with a transformed human monocytic cell line, THP-1, which has shown that exposure to physiological concentrations of retinoic acid, an active metabolite of vitamin A, induces the expression of molecules involved in antigen presentation: CD1d, an MHC-I-like molecule (implicated in lipid antigen presentation), MHC-II molecules (peptide antigen presentation), and DC-SIGN (CD209, carbohydrate antigen presentation). The data suggest that retinoic acid induces THP-1 monocytic cells to become APCs. To show that this effect of RA is not limited to transformed cells, it is important to demonstrate that normal human monocytes can be similarly regulated.