P3-ADULT BONE MARROW STEM CELLS FOR CNS REPAIR

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 来自骨髓的成人干细胞有可能为神经疾病提供戏剧性的新疗法。除了干细胞直接替代细胞外，目前的数据还提供了证据表明，成年干细胞分泌到受损的中枢神经系统环境中的神经营养因子和生长因子可能为神经系统修复提供强大的刺激。我们的工作表明，移植的骨髓干细胞的主要作用是刺激内源性中枢神经系统前体细胞在脑内的增殖。我们提出了一系列实验来验证这一中心假设，即人骨髓中的成体干细胞亚群可以通过影响内源性中枢神经系统前体细胞的增殖和分化来有效地植入和修复受损的脑组织。 具体目标是： 1.建立人骨髓中纯化的成体干细胞亚群的分子图谱，并与中枢神经系统祖细胞进行比较。 2.检测不同亚群人干细胞移植后内源性中枢神经系统祖细胞的增殖分化模式。 3.观察干细胞移植对卒中损伤动物中枢神经系统祖细胞的影响。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Adult stem cells from bone marrow have the potential to provide dramatic new therapies for neurological disorders. In addition to direct cell replacement by stem cells, current data provide evidence that neurotrophins and growth factors secreted by adult stem cells into the damaged CNS environment may provide a powerful stimulus for nervous system repair. Our work demonstrates that the primary effect of transplanted bone marrow stem cells is to stimulate the proliferation of endogenous CNS progenitors in the brain. We have proposed a series of experiments to test the central hypothesis that sub-populations of adult stem cells from human bone marrow can effectively engraft and repair the injured brain by influencing the proliferation and differentiation of endogenous CNS progenitors. The Specific Aims are: 1. To establish the molecular profiles of purified adult stem cell sub-populations from human bone marrow and compare them to CNS progenitor cells. 2. To determine the proliferation and differentiation patterns of endogenous CNS progenitors following the transplantation of different human stem cell sub-populations. 3. To evaluate the response of CNS progenitor cells following stem cell transplantation in an animal injured by stroke.