CORE B: CELL SEPARATION & ANALYSIS CORE

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 这个核心的一个总体功能是提供最先进的能力，根据物理、免疫、遗传和其他特征分析和分离细胞群体。这些能力在干细胞和祖细胞群体的工作中特别重要，但也可以在几乎所有其他类型的细胞生物学研究中找到应用。核心配备了专门的仪器，用于分析、分离或耗尽大多数类型的细胞。其中包括Becton Dickinson FACSCalibur流式细胞仪、新的FACSAria细胞分选机和Miltenyi Macs分选机(自动磁性细胞分选机)。用户是研究所范围内的，包括Cobres(Cobre血管生物学和Cobre干细胞生物学和再生医学)的研究人员。 此外，该核心正在开发为研究胚胎干细胞(ES)的资源。为干细胞研究提供技术支持、培训和服务(特别是由Zack Wang提供)。小鼠和NIH批准的人类ES细胞株将得到适当的质量保证。该设施提供的技术支持将包括胚胎干细胞的维护、转导和分化方面的实践指导。该设施还将为个人研究人员提供ES细胞的短期培养和操作，并将提供获取ES细胞试剂(RNA、基因组DNA、蛋白质)的便利途径，而不需要为ES细胞工作开发专门的技术技能(或空间)。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. One overall function of this core is to provide state-of-the-art capabilities for the analysis and separation of cell populations based on physical, immunological, genetic, and other characteristics. These capabilities are of particular importance in working with stem and progenitor cell populations, but also find application in nearly all other types of cell biology research. The Core is equipped with dedicated instruments for the analysis, isolation or depletion of most cell types. These include a Becton Dickinson FACSCalibur flow cytometer, a new FACSAria cell sorter, and a Miltenyi MACS Sorter (Automated Magnetic Cell Sorter). Users are Institute wide, and include investigators in both COBREs (COBRE Vascular Biology and COBRE Stem Cell Biology and Regenerative Medicine). In addition, this core is being developed as a resource for the study of embryonic stem (ES) cells. Technical support, training, and services for stem cell research are provided (especially by Zack Wang). Mouse and NIH-approved human ES cell lines will be maintained with appropriate quality assurance. Technical support provided by the facility will include hands-on instruction in the maintenance, transduction, and differentiation of ES cells. The facility also will provide short-term culture and manipulation of ES cells for individual investigators, and will provide ready access to ES cell reagents (RNAs, genomic DNAs, proteins) without the need to develop specialized technical skills (or space) for ES cell work.