Clinical Core

临床中心将入组并随访生殖器HSV-2和生殖器HSV-1的研究受试者。 临床核心的具体目标是：1）招募、筛选和入组研究方案受试者， 定义的人群; 2）前瞻性随访研究参与者，保持对研究的密切依从性 程序，以及3）获得病毒学和免疫学实验室所需的临床标本 项目1和项目3中详述的调查，使用标准和专门技术。特别是 努力将集中在招募a）生殖器HSV-2参与者，以实现项目1的具体目标， 包括HIV阴性、HIV阳性、孕妇和具有医源性免疫抑制的那些， 以及c）生殖器HSV-2参与者，以实现项目3的具体目标。核心还将招募 并跟踪参与研究的参与者，研究抗病毒治疗对HSV再激活的影响， HIV阴性和HIV阳性的人。对于项目2，临床中心将是新生儿 正在接受口服阿昔洛韦的患者将接受评估和监测。 核心位于华盛顿大学病毒学研究诊所（VRC）。越南革命委员会成立于 1974年，作为HSV感染的自然史和治疗学研究中心。诊所已跟进 > 1000例首次生殖器疱疹患者和> 3400例复发性HSV-2感染患者。的 临床已经率先使用每日生殖器拭子来评估粘膜上HSV的再活化， 表征HSV和HIV之间的粘膜相互作用。在过去的十年里，我们已经开始使用 HSV DNA PCR用于评价病毒脱落;使用该方法，我们已经能够证明病毒 在几乎所有HSV血清阳性的人中脱落。在我们诊所已经成为标准的其他程序 包括生殖器病变和正常皮肤活组织检查、直肠和宫颈活组织检查、生殖器的UV光诱导 复发性疱疹和白细胞分离术，用于宿主病毒的详细病毒学和免疫学研究 交互.在详细的病毒学和免疫学研究中， 储存用于将来研究HSV易感性、抗性和疾病严重程度的遗传决定因素 以及病毒测序工作。 临床中心致力于招募、筛查、登记和随访HSV患者 感染的各种研究。大多数人会随着时间的推移而被跟踪，通常每天都有拭子来测量 皮肤和粘膜上病毒脱落的频率。此外，还有各种专门技术， available.

The Clinical Core will enroll and follow study participants with genital HSV-2 as well as genital HSV-1 . Specific Aims of the Clinical Core is 1) to recruit, screen and enroll into research protocols subjects from defined populations; 2) to follow participants prospectively on studies, maintaining close adherence to study procedures, and 3) to obtain clinical specimens required for virologic and immunologic laboratory investigations detailed in Projects 1 and 3, using both standard and specialized techniques. In particular, the efforts will focus on enrolling a) participants with genital HSV-2 to address the specific aims of Project 1, including both HIV negative, HIV positive, pregnant women, and those with iatrogenic immunosuppression, and c) participants with genital HSV-2 to address the specific aims of Project 3. The core will also recruit and follow participants enrolled into studies examining the effect of antiviral therapy on HSV reactivation both in HIV negative and HIV positive persons. For project 2, the Clinical Core will be a site where the neonates who are receiving oral acyclovir will be evaluated and monitored. The Core is located at the University of Washington Virology Research Clinic (VRC). The VRC was founded in 1974 as a center for studies of natural history and therapeutics of HSV infections. The clinic has followed >1000 persons with first episode of genital herpes and >3400 persons with recurrent HSV-2 infection. The clinic has pioneered the use of daily genital swabs to evaluate the reactivation of HSV on mucosa, and to characterize the mucosal interactions between HSV and HIV. In the last decade, we have moved to using HSV DNA PCR for evaluation of viral shedding; using this method, we have been able to demonstrate viral shedding in almost all HSV seropositive persons. Other procedures that have become standard in our clinic include biopsies of genital lesions and normal skin, rectal and cervical biopsies, UV light induction of genital herpes recurrences, and leukapheresis for detailed virologic and immunologic studies of host-virus interactions. Both host and viral DNA of participants in the detailed virologic and immunologic studies are stored for future investigations of genetic determinants of HSV susceptibility, resistance and disease severity as well as viral sequencing work. The Clinical Core is dedicated to recruiting, screening, enrolling and following persons who have HSV infections into a variety of studies. Most persons are followed over time, often with daily swabs to measure the frequency of viral shedding on the skin and mucosa. In addition, a variety of specialized techniques are available.