CRYSTALLOGRAPHIC ANALYSES OF (MOSTLY) METALLOPROTEINS AND MEMBRANE PROTEINS

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 我们希望收集膜蛋白晶体的X射线衍射数据，这些数据介导了分子、电子和信息通过膜双层的过程。我们之前已经从结核分枝杆菌中确定了大电导门控机械敏感通道(MSCL)的结构，这是一种发挥渗透压安全阀功能的原核通道(Chang等人)。科学282,2220(1998))，以及当富马酸为终端电子受体时催化厌氧呼吸最后一步的富马酸还原酶(FRD)的结构(Iverson等人，科学284,1961(1999))。在此基础上，我们结晶了两个新的膜蛋白系统：MSCs和ABC转运蛋白，它们实现了分子的跨膜传递。SSRL的高强度结晶学光束线对于自然数据收集、在结构确定中使用MAD相变方法以及确定这些蛋白质上的配体结合位置将是必不可少的。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We wish to collect x-ray diffraction data from crystals of membrane proteins that mediate the passage of molecules, electrons and information across the membrane bilayer. We have previously determined the structure of the gated mechanosensitive channel of large conductance (MscL) from Mycobacterium tuberculosis, a prokaryotic channel that functions as an osmotic pressure safety valve (Chang et al. Science 282, 2220 (1998)), and the structure of fumarate reductase (FRD) that catalyzes the final step of anaerobic respiration when fumarate is the terminal electron acceptor (Iverson et al., Science 284, 1961 (1999)). Building upon this foundation, we have crystallized two new membrane protein systems, MscS and an ABC transporter, that achieve the transmembrane passage of molecules. The high intensity crystallography beamlines at SSRL will be essential for native data collection, for the use of MAD phasing methods in the structure determinations, and to identify ligand binding sites on these proteins.