CARDIAC PRESERVATION PRIOR TO TRANSPLANTATION

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 心脏移植已经成为终末期心脏病的一种挽救生命的治疗方法。供体心脏的可用性和保存心肌短暂的缺血耐受性限制了它的应用。标准冷藏将保存间隔限制在4-6小时。最新进展提示，含氧保存液灌流保存可以延长保存间隔，并允许边缘供者心脏的利用。当这项技术应用于长期储存或使用次优心脏时，其固有的局限性是很难在移植器官之前预测移植后的再灌注功能。我们之前利用心脏提取物的1H磁共振波谱(MRS)来确定在静态和灌流条件下保存的心脏之间的重要代谢差异。进行这种分析所需的组织操作和时间限制了其临床应用。魔角旋转(MAS)磁共振波谱已被用于评估完整组织的新陈代谢。我们推测~1H-MAS-MRS可用于快速判断保存心肌的代谢状态。如果成功，这将提供在临床相关的时间间隔内评估心脏保存成功的可能性。为了测试这种可能性，我们在心脏移植的大型动物模型中研究了标准静态存储和灌流存储的差异。 新陈代谢。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Heart transplantation has become a life-saving treatment for end-stage cardiac disease. Its application is limited by the availability of donor hearts and the brief ischemic tolerance of preserved myocardium. Standard cold storage limits preservation intervals to 4 - 6 hours. Recent advances suggest perfusion preservation with oxygenated storage solution may extend the storage interval and permit utilization of marginal donor hearts. An inherent limitation of this technique when applied to long-term storage or use of suboptimal hearts is the difficulty in predicting post-transplant reperfusion function prior to implanting the organ. We previously utilized 1H magnetic resonance spectroscopy (MRS) of cardiac extracts to determine important metabolic differences between hearts preserved under static and perfused conditions. The tissue manipulation and time required to perform this analysis limit its clinical application. Magic angle spinning (MAS) MRS has been used to evaluate metabolism in intact tissue. We hypothesized that 1H MAS MRS could be used to rapidly determine the metabolic status of preserved myocardium. If successful, this would offer the potential to allow assessment of cardiac preservation success in a clinically relevant time interval. To test this possibility, we studied differences between standard static storage and perfusion storage in a large animal model of cardiac transplantation." metabolism.