STAINING METHODS FOR MR HISTOLOGY OF DEVELOPING RAT AND MOUSE EMBRYO

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 该项目的总体目标是开发用于大鼠胚胎形态自动分析的策略。标本将从怀孕的Spraogue Dawley大鼠身上采集。然后，它们将按照先前研究中使用的染色方法进行修复。根据标本的大小，固定的方法是将12天、13天、14天、15天、16天、17天和18天的胎儿分别浸泡在20/1的Bouins/Prohance溶液中5分钟、10分钟、20分钟、30分钟、1小时、1.5小时和3.5小时。扫描方法将包括使用适当的线圈，以适应胚胎的大小，以尽可能地适合。将使用不同的序列获取3D图像：T1加权、T2加权和扩散加权，在高信噪比下。这些不同加权图像的组合将被用来开发大鼠胚胎的自动分割。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The global goal of this project is to develop strategies for automatic morphology analysis in the rat embryo. The specimens will be harvested from pregnant Sprague Dawley rats. They will be then fixed accordingly to the staining method used in the previous study. Depending on the size of the specimen, the fixation will be achieved by immersion in a Bouins/Prohance solution at 20/1 for 5 min, 10 min, 20 min, 30 min, 1 hr, 1.5 hrs, and 3.5 hrs for respectively 12-day, 13-day, 14-day, 15-day, 16-day, 17-day, and 18-day fetuses. The scanning method will include the use of an appropriate coil adapted to the size of the embryo to fit as well as possible. 3D images will be acquired using different sequences: T1-weighted, T2-weighted, and Diffusion-weighted, at high Signal-to-noise ratios. A combination of these different weighted images will be used to develop an automated segmentation of the rat embryo.