MOLECULAR GENETIC ANALYSIS OF FOLATE METABOLISM IN THE ZEBRAFISH(DANIO RERIO)

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 摘要：为了更好地了解叶酸在神经元发育中的作用，我们建议对斑马鱼（Danio rerio）的叶酸代谢进行分子遗传学分析。斑马鱼是一个很好的模型系统，在其中分析叶酸代谢的分子遗传学，因为它是一个脊椎动物胚胎，是适合于各种细胞，分子和遗传技术。斑马鱼的神经系统很简单，它的发育已经有了很好的描述。最重要的是，已经产生了数百种不同的斑马鱼发育突变体。克隆编码叶酸代谢关键调控酶的基因，并分析其序列和表达模式的斑马鱼突变体与NTDs应该是一个强大的方法，以更好地了解NTDs的潜在机制。这些信息应该让我们更好地了解NTD的分子遗传基础，并最终允许设计更有效的NTD治疗方法。计算机的时间提供了这笔赠款将允许在这项研究中分离的基因的序列特征，分析是绝对必要的研究的成功。研究成果-已经克隆了几个与叶酸代谢有关的基因，目前正在进行分析。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Abstract: In order to better understand the role of folic acid in neuronal development, we propose to do a molecular genetic analysis of folate metabolism in the zebrafish(Danio rerio). The zebrafish is an excellent model system in which to analyze the molecular genetics of folate metabolism, since it is a vertebrate embryo that is amenable to a variety of cellular, molecular, and genetic techniques. The nervous system of the zebrafish is simple, and its development has been well described. Most importantly, there have been hundreds of different zebrafish developmental mutants generated. Cloning the genes that encode key regulatory enzymes in folate metabolism and analyzing their sequences and expression patterns in zebrafish mutants with NTDs should be a powerful approach to better understand the underlying mechanisms of NTDs. This information should give us a much better understanding of the molecular genetic basis of NTDs, and should ultimately allow for the design of more efficacious treatments for NTDs. Computer time provided for by this grant will allow for sequence characterization of genes isolated in this study, analysis that is absolutely essential to the success of the study. Achievements - Several genes involved in folate metabolism have been cloned and are now in the process of being analyzed.