Miniaturized Wireless Implantable Biosensors for Metabolic Monitoring

描述（由申请人提供）：葡萄糖、乳酸盐O2和CO2的生理水平的变化可以提供代谢异常的早期指示，代谢异常通常随着与肥胖相关的体重增加而进行。 虽然已经确定了与肥胖相关的许多代谢趋势，但与肥胖进展和相关疾病发展相关的确切代谢物相互关系和复杂性尚不清楚。 此外，肥胖和相关的代谢变化似乎是患者特异性的，这在很大程度上是未经调查的。 由肥胖引起的代谢失衡已知会导致许多严重疾病的发展，包括糖尿病、心血管疾病、癌症和呼吸抑制。 在过去的5年里，我们的团队一直在研究一种革命性的、完全可植入的生物传感器平台，能够连续监测葡萄糖。 这种小型化（0.5 x 0.5 x 5 mm）传感器平台通过针头插入皮下，并通过个人数字助理（PDA）设备进行远程操作。 该传感器平台的关键组件之一是其涂层，其能够通过抑制炎症、纤维包裹和诱导新血管生成来控制组织/植入物界面。 拟议的研究旨在通过允许同时检测多种分析物（即葡萄糖，乳酸盐O2和CO2）来实现代谢监测的量子进步。 将建立正常和肥胖大鼠在各种生物条件（即进食、禁食和轻度运动）下这些分析物的趋势，以与我们提出的多分析物传感器进行比较。 四个电化学传感器的紧密接近需要在设备集成方面的额外进步。 在拟定的三年研究结束时，将实现第一代多分析物传感器，沿着涂层优化，以允许一个月的体内操作。 该传感器有望在代谢监测方面实现飞跃，并将为该领域的研究人员提供调查肥胖和相关疾病状态复杂性的手段。 小尺寸和低成本将增加患者的接受度和依从性，并且将允许在正常家庭环境中长时间监测大量患者。 这些数据对于该领域的研究人员来说非常有价值，有助于理解肥胖的复杂性和患者特异性。 此外，我们设想未来整合这种多分析物传感平台，以提供闭环胰岛素/胰高血糖素递送，用于严格控制糖尿病患者的葡萄糖水平。

DESCRIPTION (provided by applicant): Variations in the physiological levels of glucose, lactate O2 and CO2 can provide an early indication of metabolic abnormalities that are usually progressive with weight gain associated with obesity. Although a number of metabolic trends related to obesity have been established, exact metabolite interrelationships and complexities as they pertain to obesity progression and the development of associated diseases are less well defined. In addition, obesity and associated metabolic changes appear to be patient-specific and this is largely uninvestigated. Metabolite imbalance resulting from obesity is known to lead to the development of a number of serious diseases including diabetes, cardiovascular diseases, cancer and respiratory depression. Over the past 5 years our team has been researching a revolutionary, totally implantable biosensor platform capable of continuously monitoring glucose. This miniaturized (0.5 x 0.5 x 5 mm) sensor platform is inserted subcutaneously through a needle and operated remotely through a personal digital assistant (PDA) device. One of the key components of this sensor platform is its coating, which is capable of controlling the tissue/implant interface by suppressing inflammation, fibrous encapsulation and inducing neo-angiogenesis. The proposed research intends to make a quantum-advance in metabolic monitoring by allowing simultaneous sensing of multiple analytes (i.e. glucose, lactate O2 and CO2). The trends of these analytes under various biological conditions (i.e. fed, fasting and light exercise) for normal and obese rats will be established for comparison with our proposed multi-analyte sensor. The close proximity of the four electrochemical sensors requires additional advances in device integration. By the end of the proposed three-year study, a first generation multi-analyte sensor will be realized along with coating optimization to permit one month in vivo operation. This sensor is envisioned to achieve a quantum leap in metabolic monitoring and will provide researchers in the field with the means to investigate the complexities of obesity and associated disease states. The small size and low cost will increase patient acceptance and compliance and will allow monitoring of large numbers of patients in a normal home environment and for extended periods of time. Such data will be extremely valuable to researchers in the field to assist in understanding the complexities and patient-specific nature of obesity. Moreover, we envision future integration of this multi-analyte sensory platform to provide closed-loop insulin/glucagon delivery for tight control of glucose levels in diabetic patients.