Core C

B3.分子遗传学和表观遗传学 IHSFC的分子遗传学和表观遗传学组成部分，以前称为设施核心C： 微阵列和分子生物学，已经被改造，以满足表达的当前和未来的需求 基因表达、表观遗传学和基因研究领域的仪器和专业技术支持中心成员 功能基因组学。本活动的目标是为中心成员提供接触尖端技术的途径 通过获取仪器，指令， 在数据分析的方法和培训方面提供协助。我们已经将表观遗传学分析添加到这一点上 这项赠款的迭代，取代了以前用于蛋白质组学的资源。这一决定 是因为约翰·霍普金斯医学院的蛋白质组学核心是 (http://www.hopkinsmedicine.org/msf/)现已广泛提供给中心成员，我们选择不 重复这些努力。IHSFC这一组成部分的目标是： ？利用Affymetrix平台为小鼠提供最先进的基因表达分析和分析 (基因组430A 2.0阵列)、大鼠(Expression 230 2.0)和人类(基因组U133 Plus 2.0阵列)基因芯片 包括这些物种的全球基因图谱； ？提供基础设施，以便研究人员能够了解修改后的全球基因表达变化 通过表观遗传变化表现出来的环境暴露； ？与生物信息学/生物统计核心集成，使用BioConductor和其他工具分析 遗传和表观遗传变化。

B3. Molecular Genetics and Epigenetics The Molecular Genetics and Epigenetics component of the IHSFC, formerly called Facility Core C: Microarray and Molecular Biology, has been transformed to address the expressed current and future needs of Center members for instrumentation and expertise support in the areas of gene expression, epigenetics and functional genomics. The goal of this activity is to provide to Center members access to cutting edge technologies in the analysis of gene expression and modulation through access to instrumentation, instruction, assistance in methods and training in the analysis of data. We have added the epigenetics analysis to this iteration of the grant, replacing resources that had been previously directed towards proteomics. This decision was made because the Proteomics Core at Johns Hopkins School of Medicine is (http://www.hopkinsmedicine.org/msf/) now widely available for Center members and we choose not to duplicate these efforts. The objectives of this component of the IHSFC are to: ¿ provide state-of-the-art analysis of gene expression and analysis using the Affymetrix platform for mouse (genome 430A 2.0 array), rat (expression 230 2.0) and human (genome U133 Plus 2.0 array) gene chips that comprise the global gene profiling for these respective species; ¿ provide infrastructure so that an investigator can understand the global gene expression changes modified by environmental exposures that are manifested through epigenetic changes; ¿ integrate with the Bioinformatics/Biostatistics core for using Bioconductor and other tools for the analysis of genetic and epigenetic changes.