CORE-- Imaging and Cytometry Facilities Core

描述（由申请人提供）：成像和细胞术设施核心（ICFC）的使命是支持EHS中心成员建立的，同行评审的，资助的研究项目，为个人研究者提供很少或免费的当代技术，仪器和显微镜和流式细胞术服务。ICFC通过提供仪器、培训和必要的专业知识来提高中心成员的研究效率，以成功地将显微镜、流式细胞术和成像技术应用到他们的个人和合作研究项目中。在过去的资助期间，利用国际中心服务和设施的30名研究人员发表了83篇论文，并获得了29笔赠款（新的和续期）的资助，另有3笔赠款正在申请中，利用了国际中心使用产生的数据。获得这些技术对于从事环境、细胞和分子毒理学，特别是信号转导方面的研究至关重要。委员会在下列领域促进了多学科合作研究

Description (provided by applicant): The mission of the Imaging and Cytometry Facility Core (ICFC) is to support established, peer-reviewed, funded research programs of EHS Center members by providing access to contemporary technology, instrumentation, and services in microscopy and flow cytometry at little or no cost to individual investigators. The ICFC enhances the research productivity of Center members by providing the instrumentation, training, and expertise necessary to successfully adopt microscopic, flow cytometric, and imaging techniques into both their individual and collaborative research programs. During the past funding period, the 30 investigators utilizing ICFC services and facilities have published 83 manuscripts and received funding for 29 grants (new and renewal), with another 3 pending, utilizing data generated through ICFC use. Access to these technologies is essential for pursuing studies in environmental, cellular, and molecular toxicology, especially those in signal transduction. The ICFC has fostered multi-disciplinary collaborative research in the areas of xenobiotic effects on a variety of cell/organ systems, the effects of heavy metals on cell function and signalling, the regulation of xenobiotic metabolizing enzyme gene expression, DNA repair in response to toxic insult, regulation of cellular signalling pathways, cellular responses to oxidative stress, and T-cell phenotype association with asthma susceptibility. The ICFC are to attract new users and new Center members. Since the last competitive renewal, 16 new users, all Center members, have utilized the ICFC services/facilities. The ICFC facilitates the introduction of new approaches to investigators' individual and collaborative research programs through the provision of services requiring little initial input of time or money on the part of the investigator. The ICFC provides access to technologies and instrumentation that would otherwise be unavailable to individual investigators, and strives to introduce new technologies in response to the Center investigator needs. Advanced equipment is maintained and operated by expert core personnel, who provide service, guidance, and training to EHS Center members using the facility. Investigators can receive hands-on training in light, confocal, and fluorescent microscopy, immunocytochemistry, flow cytometry and cell sorting, and the comet assay. The ICFC also maintains equipment and instrumentation for sample analysis (e.g., spectrophotometers, fluorometer, luminometer, Agilent Bioanalyzer) and data acquisition (e.g., gel documentation system, film developer, scanning laser densitometer). The goal of the ICFC is to acquire and maintain these instruments and services in a functional multi-user environment normally unavailable to individual investigators.