COBRE: UMMC: RR CORE E: MOLECULAR BIOLOGY CORE FACILITY

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 在过去的十年里，分子生物学技术在中枢神经系统研究中的应用彻底改变了我们对突触传递、神经元可塑性、神经元整合以及精神疾病的分子基础的认识。该中心的分子生物学核心强调应用生物技术先进的分子技术来研究大脑中各种神经底物的表达、结构、功能和定位。分子生物学核心提供分子生物学方法的培训、咨询和技术援助，使研究人员能够研究各种分子终点、调节机制，以及转录和翻译过程在调节和控制各种感兴趣的神经底物表达中的作用。该核心的具体技术支持可能包括协助开发特定的分析或技术，例如：原位杂交组织化学、Northern和Southern印迹、聚合酶链式反应、RT-聚合酶链式反应、亚克隆、RNA/DNA分离、引子设计、体外转录和DNA微阵列应用。作为核心领导者，奥斯汀博士确保为CPN研究人员提供专家建议和帮助，为他们的研究项目扩大和开发分子生物学应用。 分子生物学核心提供对以下放大、可视化、检测和分析系统的共享访问： Arcturus荧光60X物镜激光捕获显微切割系统 基于软件Sw/Beacon Designer I Cycler IQ的MyiQ单色实时PCR检测系统 Biorad Gel文档系统 NanoDrop ND-1000A UV-Vis分光光度计(适用于1-2微升样品) 带有Ludl电动工作台的Zeiss Axioskop显微镜 MCID Elite图像分析系统 Axioskop数字光学显微镜工作站 热循环器 GeneQuant RNA/DNA计算器 Promega Glomax 96微板照度计 安捷伦生物分析仪 马克·奥斯汀博士是分子生物学核心的核心领导，他是一位分子神经药理学家，擅长利用各种分子生物学方法研究神经递质酶、受体、转运体和神经肽的基因表达调控。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Over the past decade, the application of molecular biological techniques to the study of the central nervous system has revolutionized our thinking about synaptic transmission, neuronal plasticity, and neuronal integration, as well as the molecular basis of psychiatric illnesses. The center's Molecular Biology Core emphasizes the application of biotechnologically advanced molecular techniques to study the expression, structure, function, and localization of a variety of neural substrates in the brain. The Molecular Biology Core provides training, consultation, and technical assistance in molecular biological approaches that allow individual researchers to study various molecular endpoints, regulatory mechanisms, as well as the role of transcriptional and translational processes in regulating and controlling the expression of the various neural substrates of interest. The specific technical support from this Core may include assisting with the development of specific assays or techniques such as: in situ hybridization histochemistry, Northern and Southern blot, PCR, RT-PCR, subcloning, RNA/DNA isolation, primer design, in vitro transcription, and DNA microarray applications. As Core Leader, Dr. Austin ensures that CPN investigators are provided expert advice and assistance to expand and develop molecular biological applications for their research programs. The Molecular Biology Core provides shared-access to the following amplification, visualization, detection and analysis systems: Arcturus Laser Capture Microdissection System with fluroescence and 60X objective MyiQ Single-Color Real-Time PCR Detection System with SW/Beacon Designer I Cycler IQ software BioRad Gel Documentation System NanoDrop ND-1000A UV-Vis Spectrophotometer (designed for 1-2 microliter samples) Zeiss Axioskop Microscope with Ludl motorized stage MCID Elite Image analysis system Axioskop Digital Photomicroscopy Workstation Thermal cycler GeneQuant RNA/DNA calculator Promega Glomax 96 microplate luminometer Agilent Bioanalyzer Dr. Mark Austin, Core Leader of the Molecular Biology Core, is a molecular neuropharmacologist with expertise in utilizing various molecular biological approaches to study the regulation of gene expression of neurotransmitter enzymes, receptors, transporters and neuropeptides.