COBRE: LSU HSC: CELL & MOLECULAR CORE

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 细胞与分子核心 Emel Songu-Mize，博士-主任 这个核心有三个主要的支持任务：细胞培养、蛋白质组学和成像。该设施的工作人员是教师杰西·古德里和M.S.奥拉·尼科尔斯。 细胞培养 核心继续为Cobre调查人员提供原代和传代细胞。这包括大鼠主动脉平滑肌细胞(使用者：Catling、Lucchesi、Wu、Songu-Mize博士)、小鼠巨噬细胞培养物(使用者：Boulares博士)、大鼠新生心肌细胞培养物(使用者：Wu博士)、HUVEC培养物(使用者：Boulares博士)。此外，细胞培养核心设施还开发了额外的小鼠肠系膜平滑肌细胞系(Lucchesi博士、Catling博士、Songu-Mize博士)、胚胎成纤维细胞(Boulares博士)。 科布雷的调查人员每周都会收到一份关于可用细胞系、传代数量、冷冻供应和蓬勃发展的培养物的报告。他们依次提出了下周的请求。尼科尔斯女士向每位调查员传达了他们所需要的信息。 蛋白质组学与成像 蛋白质组学核心通过几个不同的项目积极参与蛋白质的分析和鉴定。该核心提供基于组织和细胞实验的蛋白质样本的2DE凝胶分析。这包括交互作用组分析；筛选出与诱饵蛋白相互作用的蛋白质(Varner、Lucchesi、Catling、Lanier和Wu博士)；细胞培养处理的差异分析(Boulares、Lanier、Wu博士)；以及动物模型处理的差异分析(Boulares博士、Park博士)。 蛋白质组学核心直接提供给LSHHSC蛋白质组学核心，后者通过利用质谱学提供单独和独特的功能。通过这种密切的联系，纯质谱学申请由周文周博士处理。这些包括多肽的分离和分析(Lazartigues博士)以及蛋白质和酶靶标的修饰分析(Lanier博士)。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Cell & Molecular Core Emel Songu-Mize, Ph.D. - Director This core has three major support missions: Cell Culture, Proteomics, and Imaging. The facility is staffed by Jesse Guidry, Instructor and Olha Nichols, M.S. CELL CULTURE The core is continuing to supply primary and passaged cells for COBRE investigators. This includes rat aortic smooth muscle cells (users: Drs. Catling, Lucchesi, Wu, Songu-Mize), mouse macrophage cultures (user: Dr. Boulares), rat neonatal cardiac cell cultures (user: Dr. Wu), HUVEC cultures (user: Dr. Boulares). In addition to the above, the Cell Culture core facility developed additional cell lines of mouse mesenteric smooth muscle cells (Drs. Lucchesi, Catling, Songu-Mize), embryonic fibroblasts (Dr. Boulares). The COBRE investigators are provided a weekly report of available cell lines, passage numbers, both the frozen supplies and thriving cultures. They in turn make their request for the following week. Ms. Nichols delivers to each investigator what they need. PROTEOMICS AND IMAGING The Proteomics Core is actively involved in analysis and identification of proteins through several different projects. This core provides a 2DE gel based analysis of protein samples from tissue and cell based experiments. This includes interactome analysis; fishing out proteins that interact with bait proteins (Drs. Varner, Lucchesi, Catling, Lanier and Wu), differential analysis of cell culture treatments (Drs. Boulares, Lanier, Wu) and differential analysis of animal model treatments (Drs. Boulares, Park). The Proteomics Core feeds directly into the LSHHSC Proteomics Core which provides separate and distinct functions through the utilization of mass spectrometry. Through this close association, pure mass spectrometry applications are handled by Dr. Chau-Wen Chou. These include separation and analysis of peptides (Dr. Lazartigues) and modification analysis of proteins and enzymatic targets (Dr. Lanier).