CONSERVATION GENETICS OF FINESCALE DACE: DEVELOPMENT OF MICROSATELLITE MARKERS

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目及 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者的研究机构。 杰克米勒将构建一个基因组文库的细鳞Dace，Phoxinus neogaeus，丰富的微卫星序列。 将对该库进行筛选，以确定是否存在可用作微卫星标记的二核苷酸、三核苷酸和四核苷酸重复区域。 将对这些区域进行测序，并设计和优化对这些区域特异的PCR引物。 一旦优化的引物将可用于研究，以评估在每个位点的多态性的量和微卫星标记在未来的群体遗传学的效用。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Jake Miller will construct a genomic library for the Finescale Dace, Phoxinus neogaeus, enriched for microsatellite sequences. This library will be screened for the presence of di-, tri- and tetra-nucleotide repeat regions that may be used as microsatellite markers. Those regions will be sequenced and PCR primers specific to those regions will be designed and optimized. Once optimized the primers will be available to be used in a study to assess the amount of polymorphism at each locus and the utility of the microsatellite markers in future population genetics.