Core C- Mouse Core

Mouse Core的目的将是提供一个集中的面向服务的设施，以确保每个参与项目实验室的能力，以生产和维护转基因和基因破坏的小鼠。鼠标核心还将通过支持肺损伤模型的基础设施来服务于这些项目，该模型将被大多数项目广泛使用，即暴露在香烟烟雾中。由于大多数项目建议制造多个转基因或基因靶向的小鼠品系，并在吸烟中测试这些品系 模型，鼠标核心将是这项计划项目拨款的重要组成部分。小鼠核心的两个主要组成部分，转基因/基因敲除生产单元和吸烟设施，都由我们动物设施内的专用空间支持，并配备齐全。虽然以前不是本计划项目赠款的一部分，但该核心是由本应用程序的项目负责人Steven D.Shapiro博士于1995年在我们的肺科开发的，并已被该应用程序的项目调查人员和核心主管(高级、Shapiro、Holtzman、Parks、Shipley)广泛使用。它的工作人员在这一领域拥有广泛的专业知识和生产力的集体记录。核心已经产生了超过75个独立的转基因和基因敲除品系，每个品系都有多个创始人/嵌合体。这个核心已经和将要制造的许多鼠标将促进项目调查人员之间的协作互动。由于该计划将支持50%的转基因/基因敲除生产单位，这将由项目调查人员以及华盛顿大学的其他调查人员使用，这将使我们实现规模经济和每产生一只小鼠的较低生产成本。

The purpose of the Mouse Core will be to provide a centralized service oriented facility to insure the capacity of each participating project lab to produce and maintain transgenic and gene-disrupted mice. The Mouse Core will also serve the projects by supporting the infrastructure of a lung injury model that will be extensively utilized by most of the projects, namely exposure to cigarette smoke. As most of the projects propose to make multiple transgenic or gene-targeted lines of mice and to test these lines in the smoking model, the Mouse Core will be an important part of this Program Project Grant. The two primary components of the Mouse Core, the transgenic/knockout production unit and the smoking facility, are both supported by dedicated space within our animal facility and are fully equipped. While not previously part of this Program Project Grant, this core was developed in our Pulmonary Division in 1995 by Dr. Steven D. Shapiro, a project leader on this application, and has been extensively utilized by Project Investigators and Core Directors on this application (Senior, Shapiro, Holtzman, Parks, Shipley). It is staffed by personnel with a collective extensive track record of expertise and productivity in this area. The core has generated over 75 separate transgenic and knockout lines, with multiple founders/chimeras of each. Many of the mice which have been and will be made by this core will facilitate collaborative interactions amongst Project Investigators. As this Program would support 50% of the transgenic/knockout production unit, which will be utilized by Program Investigators as well as other investigators at Washington University, this will allow us to realize an economy of scales and lower production costs per mouse generated.