COBRE: UNR: MOLECULAR BIOLOGY CORE (B)

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 核心B是分子生物学的核心。这一核心将通过提供重组蛋白的一般克隆和表达、重组腺病毒的生产、Southern、Northern和Western分析，为所有Cobre项目服务。此外，Core B还将设计用于实时聚合酶链式反应和DNA序列分析的引物和探针，以及免疫共沉淀法的开发。此外，该核心将执行Cobre项目所需的所有准备工作，以便随后对蛋白质和蛋白质图谱进行分析和鉴定。内华达大学拥有完善的蛋白质组学设施--内华达蛋白质组学中心，该中心可以充分执行所有必要的蛋白质分析，如蛋白质质量和序列测定。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Core B is the Molecular Biology Core. This core will serve all of the cobre projects by providing general cloning and expression of recombinant proteins, recombinant adenovirus production, Southern, Northern and Western analysis. In addition, Core B will design primers and probes for real-time PCR and DNA seqeuence analysis along with co-immunoprecipitation assay development. In addition, this core will perform all preparative work required by the COBRE projects for the subsequent analysis and identification of proteins and protein profiling. The University of Nevada has a well-established proteomics facility, the Nevada Proteomics Center, that can adequately perform all the necessary protein analysis such as protein mass and sequence determination.