Task Specific Project 2

任务特定项目#2：用于术中引导的FDPM手术器械 本特定任务项目的目标是调整我们的FDPM仪器，使其适用于手术室，以定位 并鉴定由与靶向部分缀合的NIR染料描绘的癌症阳性LN，如在 主要项目。第三年，将开发第一台仪器，并交付给地中海地区气象中心使用; 第二个将在第四年的早期组装并交付BTGH使用。该工具将 设计用于快速采集荧光振幅图像，而不是断层扫描。当相位 增强器和激光二极管的增益调制之间的差保持恒定，稳态图像 对相位延迟的幅度敏感的信号被捕获。如果我们引入0度和90度相位 在调制激发光和检测器之间的变化，收集两个不同的稳态图像。 这两个图像之间的差异导致 在没有干扰的情况下获得荧光振幅图像 环境DC光水平的影响。这种方法 在荧光透视的光水平下工作， Mallinckrodt Medical和Lovelace研究所 20世纪90年代中期对犬的反应性淋巴结进行成像[90]。

Task Specific Project #2: FDPM Surgical Instrument for intraoperative guidance The goal of this Specific Task Project is to adapt our FDPM instrument for use in the surgical suite to locate and identify cancer positive LNs delineated by NIR dyes conjugated to targeting moieties as described in the primary project. In year three, the first instrument will be developed and delivered for use at the MEDVAMC; the second will be assembled and delivered for use at BTGH in the early part of year four. This instrument will be designed for rapidly acquired fluorescent amplitude images rather than tomography. When the phase difference between gain modulation of the intensifier and the laser diode is kept constant, a stead-state image is captured that is sensitive to the magnitude of the phase delay. If we introduce a zero and 90 degree phase change between the modulated excitation light and the detector, two different stead-state images are collected. The difference between these two images results in the fluorescent amplitude image is obtained without the confounding effect of the ambient DC light levels. This approach was employed under light levels of fluoroscopy with our work with Mallinckrodt Medical and the Lovelace Research Institute in the mid 1990s to image reactive lymph nodes in canines [90].