IMPRINTED GENES IN NORMAL AND ABNORMAL PLACENTAL/FETAL FUNCTION

胎儿宫内发育迟缓(IUGR)是婴儿死亡和发病的一个重要原因，影响超过 占怀孕总数的8%。与LUGR相关的并发症包括宫内死亡、分娩中胎儿窘迫和 围产期窒息。这是除了产前困难和成人更容易患上 疾病，如冠心病、高血压和糖尿病。虽然胎儿宫内发育迟缓的原因多种多样， 在相当大比例的病例中，它与胎盘有关！功能障碍。胎盘是一种复合体 执行许多功能的器官，包括营养和废物转移，除了作为一种活跃的 内分泌器官。印记基因在胎盘、胎儿和神经发育中起着重要作用。已经过去了 目前已知的75个印记基因中，有21个与胎盘特异性印记模式或 当表达异常时胎盘功能紊乱，表明它们在胎盘功能中起关键作用。 为了阐明印记基因在IUGR中的作用，我们建议对 已知印记基因在正常和IUGR胎盘中的表达。此外，我们还开发了一个模型 用于鉴定与胎盘运输功能有关的新印记基因。这些小说的表现形式 基因也将在正常和IUGR胎盘中进行研究。我们建议比较基因表达谱 单亲和双亲组织，IUGR和正常胎盘。生成的信息将被编译， 以及选定的候选基因进行进一步分析。基因表达水平和SNPs关联分析将 在IUGR和正常妊娠的胎盘中进行

Intrauterine growth retardation (IUGR) is a significant cause of infant mortality and morbidity, affecting over 8% of all pregnancies. lUGR-related complications include intrauterine death, intrapartum fetal distress, and perinatal asphyxia. This is in addition to both prenatal difficulties and increased adult predisposition to ailments such as coronary heart diseases, hypertension and diabetes. While the causes of IUGR are varied, in a significant proportion of cases it is associated with placenta! dysfunction. The placenta is a complex organ that performs many functions including nutrient and waste transfer, in addition to serving as an active endocrine organ. Imprinted genes play a major role in placenta!, fetal and neural development. Of the over 75 imprinted genes presently known, 21 are implicated in either placental-specific imprinting patterns or disruption of placental function when expressed abnormally, indicating their critical role in placental function. In order to elucidate the role of imprinted genes in IUGR we propose to undertake a detailed analysis of known imprinted gene expression in normal and IUGR placentas. In addition, we have developed a model for identifying novel imprinted genes involved in placental transport functions. The expression of these novel genes will also be studied in normal and IUGR placentas. We propose to compare gene expression profiles of uni- and bi-parental tissues, and IUGR and normal placentas. The information generated will be compiled, and candidate genes selected for further analysis. Gene expression level and SNPs association analysis will be carried out in placentas from IUGR and normal pregnancies