UNC Neuroscience Center Research Cores

描述(由申请人提供)：我们建议继续运营由NINDS机构中心核心赠款计划资助的北卡罗来纳大学神经科学中心研究中心。这些核心改变了北卡罗来纳大学教堂山NINDS资助的研究。在之前的资助期间建立了五个科学核心，以支持NINDS资助的研究人员的基因组学、遗传学和成像。每个科学核心都取得了显著的成功，这体现在有文件记载的密集和基础广泛的使用，以及基于使用核心设备和技术支持获得的数据的大量高质量出版物。NINDS受赠者之间以及NINDS受赠者和其他北卡罗来纳大学教堂山分校神经科学家之间的一些合作受到了核心服务的使用的启发。为其中三个核心建立了一个运行良好的充值系统，该系统从非NINDS用户那里完全收回成本，并为设备升级提供所需资金，这些资金不能完全由NINDS中心赠款提供。我们要求继续支持我们现有的核心：核心1：基因组和生物信息学(Affymetrix表达谱分析，SNP分析)；核心2：表达定位(原位杂交)；核心3：BAG工程技术(构建支持小鼠遗传学的载体)；核心4：胚胎干细胞技术(ES细胞电穿孔和鉴定)；核心5：共聚焦和多光子成像。我们建议通过购买新设备、实施最新研究方案、提供新服务，以及在一种情况下扩展到更多空间来改进现有的核心。此外，我们提出了一个新的核心，核心6：高通量筛查的分析开发，它将为NINDS资助的研究人员开发工具，以与北卡罗来纳大学教堂山的翻译蛋白质组学设施对接，并访问NINDS和其他NIH研究所建立的高通量筛查中心。北卡罗来纳大学神经科学中心研究核心将通过行政核心(核心7)进行管理。北卡罗来纳大学教堂山医学院完全致力于扩大神经疾病领域的研究。作为这一承诺的证据，北卡罗来纳大学教堂山医学院现在承诺提供一项实质性的一揽子计划，以支持这些核心和NINDS优先领域的新教师招聘。

DESCRIPTION (provided by applicant): We propose to continue operation of the UNC Neuroscience Center Research Cores funded by the NINDS Institutional Center Core Grant program. These Cores have transformed NINDS-funded research at UNC-Chapel Hill. Five scientific Cores were established in the prior funding period in support of genomics, genetics, and imaging for NINDS-funded investigators. Each of the scientific Cores has been a notable success as manifested by documented intense and broad based usage, and by large numbers of high quality publications based on data obtained using Core equipment and technical support. A number of collaborations among NINDS grantees and between NINDS-grantees and other UNC-Chapel Hill neuroscientists have been inspired by the use of Core services. A well-functioning recharge system is in place for three of the Cores that fully recovers costs from non-NINDS users and provides needed funds for equipment upgrades that cannot be fully funded by this NINDS Center grant. We request continued support of our existing Cores: Core 1: Genomics and Bioinformatics (Affymetrix expression profiling, SNP analysis); Core 2: Expression Localization (in situ hybridization); Core 3: BAG Engineering Technology (vector construction in support of mouse genetics); Core 4: ES Cell Technology (ES cell electroporation and characterization) and Core 5: Confocal and Multiphoton Imaging. We propose to improve our existing Cores with new equipment purchases, implementation of the latest research protocols, provision of new services, and in one case expansion into additional space. In addition, we propose a new Core, Core 6: Assay Development for High Throughput Screening that will develop tools for NINDS-funded investigators to interface with the Translational Proteomics Facility at UNC-Chapel Hill and to access High Throughput Screening Centers established by NINDS and other NIH institutes. The UNC Neuroscience Center Research Cores will be managed via an Administrative Core (Core 7). The UNC-Chapel Hill School of Medicine is fully committed to expansion of research in the area of neurological disease. As evidence of this commitment, the UNC-Chapel Hill School of Medicine now commits a substantial package in support of these Cores and in support of new faculty hires in NINDS priority areas.