Genetic Core

对小鼠模型的遗传操作一直是研究基础知识的一种非常有成效的方法 生物机制。遗传学核心已经建立，并在过去的几年中取得了丰硕的成果 资助期。RDCC建议继续支持Genetics Core提供两项主要服务： 1)转基因和基因敲除小鼠的生产；2)快速基因工程。转基因和基因敲除小鼠 生产设施提供额外的服务，包括囊胚补充，新的 胚胎干细胞系，冷冻保存，小鼠胚胎成纤维细胞生产。速度 基因设备提供全基因组分型，目的是加强小鼠回交到 明确的遗传背景。因此，该设施将加强对基本机制的研究。 风湿病。

Genetic manipulation of mouse models has been an extremely productive approach to studying basic biological mechanisms. A Genetics Core has been established and has been productive during the last funding period. The RDCC proposes to continue support of the Genetics Core to provide two major services: 1) Transgenic and knockout mouse production and 2) Speed congenics. The transgenic and knockout mouse production facility provides additional services including blastocyst complementation, derivation of new embryonic stem cell lines, cryopreservation, and mouse embryonic fibroblast production. The speed congenics facility provides genome wide typing for the purposes of enhancing backcrossing of mice into defined genetic backgrounds. Thus, the facility will enhance the study of basic mechanisms underlying rheumatic diseases.