Gene Targeting & Transgenics

但前提是。 在过去的十年里，转基因小鼠越来越多地成为许多新的 癌症模型帮助阐明了促进肿瘤发展和进展的途径。 随着人类和小鼠基因组序列的完成和大规模努力的启动 从功能上诠释这些基因组，这一趋势将继续加速。从基因上生产 改良的小鼠需要高度专业化的仪器和大多数实验室所没有的专业知识。这个 基因打靶和转基因资源的任务是确保RPCI的研究人员 获得最先进的转基因小鼠技术、方法和动物模型。《资源》 主任和资源助理从最早的规划阶段为调查人员提供指导 在表型分析期间，当构造被设计到项目的高级阶段时，项目。 资源技术人员执行专门的ES细胞和胚胎操作方法以生成 转基因小鼠。该资源有几个核心载体，它们已在 制备修饰的小鼠，这些载体可根据需要自由分发给用户。新资源 服务包括使用BAG DMA准备转基因小鼠，基于ES细胞的转基因，DNA 纯化服务、小鼠品系冷冻保存和定制组织培养服务。也是 在过去的几年里，已经实施了质量控制倡议，如测试DMA和 用于毒性和细胞系污染物和完整性测试的介质。到目前为止，该资源已 提供了516个新的转基因品系(从165个构造中敲除79个和437个转基因)用于 四个CCSG计划。它还帮助开发了独特的老鼠模型，这些模型有助于 了解印记及其在癌症中的作用、重要基因的调控和两种转基因模型 它模仿与特定人类癌症相关的染色体重排。资源的使用具有 随着遗传学、细胞应激和生物物理疗法的不断招募， 前列腺和分子靶点以及实验治疗计划和项目需要 转基因小鼠模型的建立。预计资源使用量将继续增加。 该资源由四个项目使用，55%的用户是CCSG成员。CCSG中的36,596美元 请求提供支助，占业务预算总额的13%。

PROVIDED. During the past decade, genetically modified mice have increasingly been a source of many new cancer models that have helped elucidate pathways contributing to tumor development and progression. With the completion of the human and mouse genome sequences and the initiation of large-scale efforts to functionally annotate these genomes, this trend will continue to accelerate. Production of genetically modified mice requires highly specialized instrumentation and expertise not available in most labs. The mission of the Gene Targeting and Transgenics Resource is to ensure that investigators at RPCI have access to state-of-the-art transgenic mouse technologies, methods and animal models. The Resource Director and Resource Assistant provide guidance to investigators from the earliest planning stages of the project when constructs are designed to advanced stages of the project during phenotype analysis. Resource technicians perform the specialized ES cell and embryo manipulation methods to generate the genetically modified mice. The Resource has several core vectors that have been successfully used in the preparation of modified mice, and these vectors are freely distributed to users as needed. New Resource services include preparation of transgenic mice using BAG DMA, ES cell-based transgenesis, a DNA purification service, cryopreservation of mouse strains and custom tissue culture services. Also implemented over the last several years have been quality control initiatives such as testing of DMAs and media for toxicity and testing of cell lines for contaminants and integrity. To date, the Resource has provided 516 new genetically modified lines (79 knockouts and 437 transgenics from 165 constructs) for four CCSG Programs. It also has assisted in the development of unique mouse models that contribute to understanding imprinting and its role in cancer, regulation of important genes and two transgenic models that mimic chromosome rearrangements associated with specific human cancers. Use of the Resource has continued to increase with the ongoing recruitment into the Genetics, Cell Stress and Biophysical Therapies, Prostate and Molecular Targets and Experimental Therapeutics Programs and projects requiring the development of transgenic mouse models. Increased usage of the Resource is expected to continue. The Resource is used by four Programs and 55% of users are CCSG members. $36,596 in CCSG support is requested, representing 13% of the total operating budget.