Antidiabetic Constituents from the Dominican Medicinal Plant Momordica charantia

描述（由申请人提供）：苦瓜是一种广泛用于治疗糖尿病的传统多米尼加医学的植物。本项目假设从M.苦瓜果实通过增加β-细胞复制和功能降低血糖浓度，从而改善胰岛素分泌。为了验证这一假设，M.将提取、分级分离Charantia，并用β-细胞测定法进行测试，以鉴定活性级分。将使用各种色谱方法从活性级分中分离生物活性化合物。分离后，将使用现代植物化学技术鉴定活性化合物，并使用β-细胞在体外进一步测试，以量化对作为参考的已建立的口服降血糖药物的作用。根据其在β-细胞系中的活性、化学结构的独特性和产率，选择两种生物活性化合物进行体内试验。然后将在肥胖诱导的高血糖症的小鼠模型中测试所选化合物沿着用于II型糖尿病的常用处方药物的阳性对照，以在功能上研究化合物的降血糖活性。此外，从果实中分离的活性化合物将在M的茎和叶中定量。charantia，因为这些植物的部分被用于传统的多米尼加医学。许多传统疗法在科学和医学界没有得到严格的研究。因此，该项目将提供植物化学技术和研究方面的培训，以及草药科学研究所需技能方面的培训。这种跨学科的培训对于补充和替代医学的严格科学研究中的终身职业目标至关重要。苦瓜是一种广泛用于治疗世界上最具威胁性的疾病之一糖尿病的植物。虽然这种植物的抗糖尿病活性已被研究，很少有研究严格测试的生物活性M。charantia化合物的具体作用机制。这项研究的结果将使人们能够对M的使用做出更明智的决定。Charantia治疗糖尿病

DESCRIPTION (provided by applicant): Momordica charantia is a widely-used plant in traditional Dominican medicine for diabetes treatment. This project hypothesizes that the active compounds isolated from M. charantia fruits lower blood glucose concentrations via an increase in (3-cell replication and function, resulting in improved insulin secretion. To test this hypothesis, the fruits of M. charantia will be extracted, fractionated, and tested with a p-cell assay in order to identify the active fractions. Various chromatographic methods will be used to isolate the bioactive compounds from the active fractions. After isolation, the active compounds will be identified using modern phytochemical techniques and further tested in vitro using (3-cells to quantify the effects against an established oral hypoglycemic drug as a reference. Two of the bioactive compounds will be selected for in vivo testing based on their activity in the (3-cell line, uniqueness of chemical structure, and yield. The selected compounds will then be tested in a mouse model of obesity-induced hyperglycemia along with positive controls of commonly prescribed medications for Type II diabetes mellitus to functionally investigate the compounds' hypoglycemic activity. In addition, the active compounds isolated from the fruit will be quantified in the stems and leaves of M. charantia, as these parts of the plants are used in traditional Dominican medicine. Many traditional remedies have not been rigorously researched in the scientific and medical communities. Therefore, this project will provide training both in phytochemical techniques and research, and also in the skills necessary for the scientific research of herbal medicine. This interdisciplinary training is vital to a goal of a lifetime career in the rigorous scientific study of complementary and alternative medicine. Momordica charantia is a widely-used plant in treating one of the world's most threatening diseases, diabetes. Although this plant's antidiabetic activity has been researched, very few studies have rigorously tested the bioactive M. charantia compounds for specific mechanisms of action. The results of this study will allow people to make more informed decisions about the use of M. charantia in diabetes treatment.