PHYSIOLOGIC EFFECTS OF GENETIC VARIATION IN TRANSCRIPTION FACTOR 7-LIKE 2: (B

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 这是一项基于基因型的回忆研究，旨在对比转录因子7-样蛋白2(TCF7L2)基因中特定单核苷酸多态(SNP)不一致的个体。TCF7L2是2型糖尿病的易感基因，可能会影响胰岛β细胞功能。这项研究将召回之前参与Beta基因研究的不符合SNP rS 12255372的参与者，并重复最初的Beta基因研究访问中进行的一系列表型分析。本研究的目的是比较两种基因型组之间的差异，并评估口服葡萄糖耐量试验(OGTT)期间GLP1浓度的差异。第二个目标是评估表型的时间变化(当前表型值-最初访问Betegene时的表型值)在两种基因型之间是否不同，以及这些变化是否被体脂改变。以前参与Betegene研究的非糖尿病患者与rs12255372基因不一致的人将有资格参加这项研究。参加者将接受两次GCRC访问。第一次访问将包括体检和口服葡萄糖耐量试验。第二次检查将包括静脉葡萄糖耐量测试和DEXA扫描。。将采集空腹血液进行DNA、RNA和血脂检测。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This is a genotype-based recall study to contrast individuals discordant for specific single nucleotide polymorphism (SNP) in the gene for transcription factor 7-like 2 (TCF7L2). TCF7L2 is a susceptibility gene for type 2 diabetes that appers to affect pancreatic beta-cell function.This study will recall pervious participants of the BetaGene study who are discordant for the SNP rs 12255372 and repeat the series of phenotyping that was performed in the original BetaGene study visit. The purpose of this study it to contrast between the two genotypes groups and to also assess differences in GLP1 concentrations during the oral glucose tolerance test (OGTT). The second goal is to assess whether the temporal change in the phenotypes (current phenotype value - phenotype value at original BeteGene visit ) is different between the two genotypes and whether these changes are altered by body fat. Non-diabetic individuals who previously participated in the BeteGene study are discordant for rs12255372 genotype will be eligible for this study. Paticipants will undergo two GCRC visits. The first visit will consist of physical examination and OGTT. The second visit will consist of an intravenous glucose tolerance test and a DEXA scan. . Fasting blood will be collected for DNA, RNA, and lipids.