MAINTENANCE AND REPAIR OF THE IVEM F30

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 在2006年4月1日至2007年4月30日的报告期内，F30显微镜已用于生成1414个倾斜系列，尽管由于更换HT槽而停机64小时，以及为Ultracam安装新芯片而停机35小时。105个小时用于软件和硬件开发，其中大部分是由我们的SerialEM研讨会的建议直接引起的。F30每周使用36小时（2017.25小时，56周）。从2006年9月1日起，我们开始通过打卡和打卡来记录工作时间，而不仅仅是记录总工作时间。从这些数据中，我们已经确定，大约24%的显微镜时间是在几个小时后（下午5点-上午8点M-F和周末）。图1显示了基于总使用小时数的F30使用情况。塑料和冷冻成像是专门用于检查样本和映射区域的会议，以便以后进行断层扫描。培训专门的冷冻课程和在SerialEM研讨会上花费的时间。图2显示了报告期间在F30上进行的不同类型断层扫描工作的划分：室温下塑料包埋切片（塑料TS）的单轴或双轴断层扫描，包括剪辑数据集。将导联组合视为单帧倾斜系列的总和，即如果是2X2导联组合，则计为4个倾斜系列，冷冻断层扫描（冷冻TS）耗时更多，因此拍摄的倾斜系列较少。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. In the reporting period of 4/1/06-4/30/07, the F30 microscope has been used to generate 1414 tilt-series, despite being down for 64 hours due to the replacement of the HT tank and 35 hours for the installation of a new chip for the Ultracam. 105 hours was spent on software and hardware development, much of which was directly caused by suggestions from our SerialEM workshop. The F30 was in use 36 hours/week (2017.25 hours for 56 weeks). From September 1, 2006, we started keeping track of hours by clocking in and out vs. just total hours logged. From that data, we have determined that about 24% of microscope time is after hours (5pm-8am M-F and weekends). Figure 1 illustrates the F30 usage based on the total number of hours used. Plastic and cryo imaging are sessions devoted to checking samples and mapping areas for later tomography. Training specialized cryo-sessions and time spent on the SerialEM Workshop. Figure 2 shows the division of different types of tomography work conducted on the F30 during the reporting period: single or dual-axis tomography of plastic-embedded sections at room temperature (Plastic TS) including montaged data sets. A montage was treated as a sum of single-frame tilt-series i.e. if it was a 2X2 montage, it was counted as 4 tilt-series, Cryo tomography (Cryo TS) is considerably more time consuming hence there were less tilt-series taken.