URINARY STEROID PROFILES AND DIETARY SUPPLEMENTS

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 普通公众和精英运动员对不受管制的膳食补充剂的可获得性和使用迅速增加。 这些产品在体育界的使用程度在很大程度上是未知的。 然而，已经证明这些产品中的许多含有雄激素合成代谢类固醇（AAS）、类固醇激素原（前体）或类固醇样化合物。 这些化合物的意外口服摄入在体育界越来越受到关注，因为服用保健品的人可能会在药物测试中产生“阳性”的尿液。 此外，尚未研究这些暴露产生的代谢物的典型尿液特征，也不知道这会如何影响检测过程。 本提案的目的是表征暴露于选定的非生理性类固醇和激素原后的独特和动态人尿类固醇代谢产物谱，这些类固醇和激素原以以下方式给药：1）单次给药，和2）最多5天单次给药。 这些产品不会长期给药。 为实现这一目标，该项目有以下目标： 1. 确定在选定的膳食补充剂中销售的几种非生理性类固醇和激素原单次给药后代谢物消除的独特特征和时间过程。 2. 确定在选定的膳食补充剂中销售的几种非生理性类固醇和激素原多次给药（5天）后代谢物消除的独特特征和时间过程。 3. 比较尿液代谢物的轮廓和消除的时间过程，结果从暴露于不同配方的几个非生理类固醇和激素原出售选定的膳食补充剂。 这些具体目标的假设是： 1. 在给予选定的类固醇激素原和选定的膳食补充剂中发现的前体后，内源性尿类固醇特征不会发生变化。 2. 在给予选定的类固醇激素原和选定的膳食补充剂中发现的前体后，睾酮/表雄激素没有变化。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. There has been a rapid rise in the availability and use of unregulated dietary supplements by the general public and also by elite athletes. The extent of use of these products within the sports community is largely unknown. However, it has been demonstrated that many of these products contain androgenic-anabolic steroids (AAS), steroid prohormones (precursors), or steroid-like compounds. The unsuspected oral ingestion of these compounds is a growing concern in the sports community because individuals who consume health supplements may produce urine that is "positive" upon drug testing. Also, the typical urine profiles of metabolites produced as a result of these exposures has not been studied and it is unknown how this might affect the testing process. The goal of this proposal is to characterize the unique and dynamic human urinary steroid metabolite profile(s) after exposure to selected non-physiologic steroids and prohormones administered as: 1) a single dose, and 2) a maximum of five days of single doses. No long term administration of these products will be undertaken. To achieve this goal, this project has the following objectives: 1. Determine the unique profile and time course of elimination of metabolites after single-dose administration of several non-physiologic steroids and prohormones sold in selected dietary supplements. 2. Determine the unique profile and time course of elimination of metabolites after multiple-dose administration (5 days) of several non-physiologic steroids and prohormones sold in selected dietary supplements. 3. Compare the urine metabolite profiles and time course of elimination that results from exposure to different formulations of several non-physiologic steroids and prohormones sold in selected dietary supplements. The hypotheses for these specific aims are: 1. There will be no change in endogenous urine steroid profile after administration of selected steroid prohormones and precursors found in selected dietary supplements. 2. There will be no change in the testosterone/epitestosterone ration after administration of selected steroid prohormones and precursors found in selected dietary supplements.