HUMAN URINARY STEROID PROFILES AFTER EXPOSURE TO NON-PHYSIOLOGIC STEROIDS

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。普通公众和精英运动员获得和使用不受管制的膳食补充剂的情况迅速增加。这些产品在体育界的使用程度在很大程度上是未知的。然而，已经证明，这些产品中的许多含有雄激素合成代谢类固醇(AAS)、类固醇前体激素(前体)或类固醇类化合物。这些化合物的意外口服在体育界引起了越来越多的关注，因为服用保健品的人可能会产生药检呈阳性的尿液。此外，由于这些暴露而产生的代谢物的典型尿液特征还没有被研究，也不知道这可能如何影响检测过程。这项建议的目标是描述在接触选定的非生理性类固醇和前激素后，人类独特和动态的类固醇代谢物谱(S)：1)单剂，2)最多5天的单剂。不会对这些产品进行长期管理。为了实现这一目标，本项目有以下目标：1.确定在选定的膳食补充剂中销售的几种非生理性类固醇和前激素单剂量给药后代谢物消除的独特情况和时间过程。2.确定在选定的膳食补充剂中出售的几种非生理性类固醇和前激素在多剂量(5天)给药后，代谢物消除的独特情况和时间过程。3.比较在选定的膳食补充剂中销售的几种非生理性类固醇和前激素的不同配方所导致的尿代谢物分布和排出时间。这些特定目的的假设是：1.在给予选定的类固醇前体和在选定的膳食补充剂中发现的类固醇前体后，内源性尿类固醇的特征不会改变。2.在服用选定的类固醇前体激素和在选定的膳食补充剂中发现的前体后，睾酮/表雄酮比率不会发生变化。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. There has been a rapid rise in the availability and use of unregulated dietary supplements by the general public and also by elite athletes. The extent of use of these products within the sports community is largely unknown. However, it has been demonstrated that many of these products contain androgenic-anabolic steroids (AAS), steroid prohormones (precursors), or steroid-like compounds. The unsuspected oral ingestion of these compounds is a growing concern in the sports community because individuals who consume health supplements may produce urine that is "positive" upon drug testing. Also, the typical urine profiles of metabolites produced as a result of these exposures has not been studied and it is unknown how this might affect the testing process. The goal of this proposal is to characterize the unique and dynamic human urinary steroid metabolite profile(s) after exposure to selected non-physiologic steroids and prohormones administered as: 1) a single dose, and 2) a maximum of five days of single doses. No long term administration of these products will be undertaken. To achieve this goal, this project has the following objectives: 1. Determine the unique profile and time course of elimination of metabolites after single-dose administration of several non-physiologic steroids and prohormones sold in selected dietary supplements. 2. Determine the unique profile and time course of elimination of metabolites after multiple-dose administration (5 days) of several non-physiologic steroids and prohormones sold in selected dietary supplements. 3. Compare the urine metabolite profiles and time course of elimination that results from exposure to different formulations of several non-physiologic steroids and prohormones sold in selected dietary supplements. The hypotheses for these specific aims are: 1. There will be no change in endogenous urine steroid profile after administration of selected steroid prohormones and precursors found in selected dietary supplements. 2. There will be no change in the testosterone/epitestosterone ration after administration of selected steroid prohormones and precursors found in selected dietary supplements.