EFFECT OF INTRAVENOUS ASCORBIC ACID ON OXIDATIVE STRESS AND CYTOKING ACTIVATION

这个子项目是许多利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 该提案将研究中心假设：AA补充将急性增强细胞因子激活和氧化应激产生的静脉内铁通过增加血液透析（HD）患者的NTBI浓度。 具体目标 1)确定在NTBI存在下AA对细胞因子活化、DNA、脂质和蛋白质氧化的影响。 2)目的：研究AA与NTBI出现幅度的关系。 3)评价HD患者中的临床相关预测因素以及AA和Fe诱导的氧化应激和炎症。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This proposal will examine the central hypothesis that: AA supplementation will acutely potentiate cytokine activation and oxidative stress produced by intravenous Fe by increasing NTBI concentrations in hemodialysis (HD) patients. Specific Aims 1) To determine the effect of AA in the presence of NTBI on cytokine activation, DNA, lipid, and protein oxidation. 2) To study the relationship between AA and magnitude of NTBI apparance. 3) To evaluate clinically-revelant predictors and AA and Fe-induced oxidative stress and inflammation in HD patients.